Bagheri-Josheghani Sareh, Bakhshi Bita
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Can J Infect Dis Med Microbiol. 2022 Mar 23;2022:3432235. doi: 10.1155/2022/3432235. eCollection 2022.
is a major cause of severe diarrhea, which is ecologically flexible, and remains as a major cause of death, especially in developing countries. Consecutive emergence of antibiotic-resistant strains is considered to be as one of the major concerns of the World Health Organization (WHO). Nanoparticles as a new nonantibiotic therapeutic strategy have been widely used in recent years to treat bacterial infections. The present study aimed to investigate the antibacterial and antibiofilm effect of selenium nanoparticles (SeNPs) in vitro against O1 ATCC 14035 strain. SeNPs were prepared and characterized using ultraviolet-visible (UV-Vis) spectroscopy, DLS (dynamic light scattering), zeta potential measurement, and Fourier transform infrared (FTIR) analysis. The concentration of SeNPs was calculated by ICP (inductively coupled plasma) method. Also, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was employed to assess the cytotoxic effect of SeNPs on Caco-2 cells. Antibacterial and antibiofilm activity of SeNPs was determined by broth microdilution and crystal violet assays, respectively. The average particle size of SeNPs was 71.1 nm with zeta potential -32.2 mV. The SEM images supported the uniform spherical morphology of the prepared nanoparticles. The antibiofilm effect of SeNPs was evident at concentrations of 50-200 g/mL. This study results provided evidence that SeNPs are safe as an antibacterial and antibiofilm agent against O1 ATCC 14035 strain.
是严重腹泻的主要原因，其生态适应性强，仍是主要死因，尤其是在发展中国家。抗生素耐药菌株的连续出现被认为是世界卫生组织（WHO）主要关注的问题之一。近年来，纳米颗粒作为一种新的非抗生素治疗策略已被广泛用于治疗细菌感染。本研究旨在体外研究硒纳米颗粒（SeNPs）对O1 ATCC 14035菌株的抗菌和抗生物膜作用。使用紫外可见（UV-Vis）光谱、动态光散射（DLS）、zeta电位测量和傅里叶变换红外（FTIR）分析对SeNPs进行制备和表征。通过电感耦合等离子体（ICP）法计算SeNPs的浓度。此外，采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴盐（MTT）试验评估SeNPs对Caco-2细胞的细胞毒性作用。分别通过肉汤微量稀释法和结晶紫试验测定SeNPs的抗菌和抗生物膜活性。SeNPs的平均粒径为71.1 nm，zeta电位为-32.2 mV。扫描电子显微镜（SEM）图像支持所制备纳米颗粒的均匀球形形态。SeNPs在浓度为50-200μg/mL时抗生物膜作用明显。本研究结果提供了证据，表明SeNPs作为针对O1 ATCC 14035菌株的抗菌和抗生物膜剂是安全的。
