Satpathy Siddharth, Panigrahi Lipsa Leena, Samal Pallavi, Sahoo Kirti Kanta, Arakha Manoranjan
Centre for Biotechnology, Siksha 'O' Anusandhan (Deemed to Be University), Bhubaneswar, 751003, Odisha, India.
School of Civil Engineering, Kalinga Institute of Industrial Technology Univ., Bhubaneswar, Odisha, 751024, India.
Heliyon. 2024 Jun 8;10(12):e32499. doi: 10.1016/j.heliyon.2024.e32499. eCollection 2024 Jun 30.
Biogenic synthesis of nanoparticles has been established as an environmentally benign and sustainable approach. This study emphasizes biosynthesis of selenium nanoparticles (SeNPs) utilizing leaf extract of L., well known for its abundant bioactive compounds. Various analytical techniques were employed for characterization of synthesized SeNPs. X-ray diffraction (XRD) spectroscopy confirmed the crystalline structure and revealed the average crystalline size of SeNPs to be 44.57 nm. Additionally, UV-Vis spectroscopy confirmed successful synthesis of SeNPs by validating the surface plasmon resonance (SPR) properties of SeNPs. FTIR analysis data revealed different bonds and their corresponding functional groups responsible for the synthesis and stability of synthesized SeNPs. DLS and zeta analysis revealed that 116.5 nm sized SeNPs were stable in nature. Furthermore, field emission scanning electron microscopy (FE-SEM) validated the spherical morphology of SeNPs with a size range of 60-80 nm. Inductively coupled plasma-optical emission spectroscopy (ICP-OES) determined the concentration of SeNPs in the obtained colloidal solution. Antioxidant activity of synthesized SeNPs was evaluated employing DPPH and HO assay, revealed that the synthesized SeNPs were effective antioxidant agent. Additionally, antimicrobial potential was evaluated against a panel of Gram-positive and Gram-negative bacteria and found to be effective at higher concentration of SeNPs. SeNPs also exhibited strong anti-biofilm activity while evaluated against various biofilm producing bacteria like and . The cytotoxicity of the bio-synthesized SeNPs was evaluated against HEK 293 cell line, exhibited minimal toxicity even at concentration 100 μg/mL with 65% viable cells. SeNPs has also been evaluated for dye degradation which has indicated excellent photocatalytic activity of synthesized SeNPs. The experimental data obtained altogether demonstrated that synthesized SeNPs exhibited significant antimicrobial and anti-biofilm activity against various pathogens, and also showed significant antioxidant and photocatalytic efficiency.
纳米颗粒的生物合成已被确立为一种环境友好且可持续的方法。本研究着重于利用富含多种生物活性化合物的[植物名称]叶提取物生物合成硒纳米颗粒（SeNPs）。采用了各种分析技术对合成的SeNPs进行表征。X射线衍射（XRD）光谱证实了其晶体结构，并显示SeNPs的平均晶体尺寸为44.57纳米。此外，紫外可见光谱通过验证SeNPs的表面等离子体共振（SPR）特性，证实了SeNPs的成功合成。傅里叶变换红外光谱（FTIR）分析数据揭示了负责合成的SeNPs及其稳定性的不同化学键及其相应的官能团。动态光散射（DLS）和zeta电位分析表明，尺寸为116.5纳米的SeNPs在性质上是稳定的。此外，场发射扫描电子显微镜（FE-SEM）验证了SeNPs的球形形态，尺寸范围为60-80纳米。电感耦合等离子体发射光谱（ICP-OES）测定了所得胶体溶液中SeNPs的浓度。采用二苯基苦味酰基自由基（DPPH）和羟基自由基（HO）测定法评估了合成的SeNPs的抗氧化活性，结果表明合成的SeNPs是有效的抗氧化剂。此外，还针对一组革兰氏阳性和革兰氏阴性细菌评估了其抗菌潜力，发现其在较高浓度的SeNPs时有效。在针对各种产生物膜细菌如[细菌名称1]和[细菌名称2]进行评估时，SeNPs还表现出很强的抗生物膜活性。对生物合成的SeNPs针对人胚肾293（HEK 293）细胞系的细胞毒性进行了评估，结果表明即使在浓度为100μg/mL时，其毒性也极小，仍有65%的活细胞。还对SeNPs进行了染料降解评估，结果表明合成的SeNPs具有优异的光催化活性。总共获得的实验数据表明，合成的SeNPs对各种病原体表现出显著的抗菌和抗生物膜活性，并且还显示出显著的抗氧化和光催化效率。
