Azimi Mohammadreza, Mehrzad Jalil, Ahmadi Elnaz, Orafei Mohammad, Aghaie Fatemeh, Ahmadi Armita, Rahimi Maryam, Ghorbani Ranjbary Ali
Department of Biochemistry, Medical Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Evid Based Complement Alternat Med. 2022 Mar 23;2022:2948966. doi: 10.1155/2022/2948966. eCollection 2022.
Many diseases, including diabetes, are involved in the development of liver disorders through changes in the expression of genes such as apoptosis-related genes. In the present study, the effect of (. ) on hepatic enzyme activity and apoptosis-related gene expression in streptozotocin (STZ)-induced diabetic rats was examined. In this study, 50 adult male Wistar rats weighing approximately 200-220 g were divided into five groups. Diabetes was induced by an intraperitoneal injection of STZ (60 mg/kg). Following 18 days, all the animals in different groups were weighed and blood samples were taken from their cardiac veins. Gas chromatography-mass spectrometry (GC-MS) analysis revealed 45 different compounds in the . , including thymol (39.1%), p-cymene (20.63%), and -terpinene (14.85%). The results showed a significant increase in liver enzymes (aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP)) in diabetic or STZ mice compared to the control group (healthy mice) ( < 0.0001). The levels of AST, ALT, and ALP in rats treated with 200 mg/kg and 400 mg/kg of . extract showed a significant decrease in these enzymes in comparison with diabetic rats ( < 0.0001). The expression of caspase 3 and 9 genes in the groups treated with thyme significantly decreased compared to diabetic mice ( < 0.0001), and the expression of B-cell lymphoma-2 (Bcl-2) in the group receiving 400 mg/kg of thyme significantly increased compared to diabetic mice ( < 0.0001). Due to its antioxidant compounds, thyme improves the liver tissue cells in STZ-induced diabetic mice by reducing caspases 3 and 9 as well as increasing Bcl-2.
许多疾病，包括糖尿病，通过凋亡相关基因等基因表达的变化参与肝脏疾病的发展。在本研究中，检测了（.）对链脲佐菌素（STZ）诱导的糖尿病大鼠肝酶活性和凋亡相关基因表达的影响。在本研究中，将50只体重约200 - 220克的成年雄性Wistar大鼠分为五组。通过腹腔注射STZ（60毫克/千克）诱导糖尿病。18天后，对不同组的所有动物进行称重，并从其心脏静脉采集血样。气相色谱 - 质谱（GC - MS）分析显示（.）中存在45种不同的化合物，包括百里香酚（39.1%）、对伞花烃（20.63%）和γ - 萜品烯（14.85%）。结果显示，与对照组（健康小鼠）相比，糖尿病或STZ小鼠的肝酶（氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP））显著升高（<0.0001）。用200毫克/千克和400毫克/千克的（.）提取物处理的大鼠中，AST、ALT和ALP水平与糖尿病大鼠相比，这些酶显著降低（<0.0001）。与糖尿病小鼠相比，用百里香处理的组中半胱天冬酶3和9基因的表达显著降低（<0.0001），接受400毫克/千克百里香的组中B细胞淋巴瘤 - 2（Bcl - 2）的表达与糖尿病小鼠相比显著增加（<0.0001）。由于其抗氧化化合物，百里香通过降低半胱天冬酶3和9以及增加Bcl - 2来改善STZ诱导的糖尿病小鼠的肝组织细胞。
