Wei Kang, Guo Congyin, Zhu Jiangxiong, Wei Yang, Wu Meirong, Huang Xiaodong, Zhang Mu, Li Jide, Wang Xueyun, Wang Yuanfeng, Wei Xinlin
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
College of Life Sciences, Shanghai Normal University, Shanghai, China.
Front Nutr. 2022 Mar 18;9:837168. doi: 10.3389/fnut.2022.837168. eCollection 2022.
Selenium-enriched mung bean (Se-MB) is a combination of mung bean (MB) and selenium (Se), which have a variety of potential biological activities. However, little is known about the skincare activity of Se-MB. The chemical composition of Se-MB fermentation broth (Se-MBFB) was analyzed to investigate the whitening, moisturizing, and anti-aging activities of Se-MBFB. The tyrosinase inhibition, anti-melanogenic in melanocytes (B16F10 cells), and moisturizing effect in human dermal fibroblasts (HDFs) were analyzed. Besides, the free radical scavenging activity of Se-MBFB was assessed . To verify the effects and the potential of practical applications of Se-MBFB, a clinical trial was conducted on the participants: 31 Chinese women aged 25-60 years, with no pigmentation disorder, no illness, no history of hypersensitivity reaction, and no use of skincare product on the face. The participants used an Se-MBFB masque for 15-20 min after cleaning the face. The measurement points were Week 0, 2, and 4 (W0, W2, and W4) after using the masque, and target sites were cheek and canthus. The following parameters were recorded on the target sites at each visit: melanin index, skin color, cuticle moisture content, transepidermal water loss, and crow's feet. The results demonstrated that Se-MBFB was rich in polyphenols, peptides, and γ-aminobutyric acid (GABA), displayed significant free radical scavenging and tyrosinase inhibiting activities, decreased the synthesis of melanin, and upregulated the aquaporin-3 (AQP3) expression. The test of the Se-MBFB mask showed that after 4 weeks of using the Se-MBFB facemask, the faces of the participants became whiter with reduced wrinkles and increased moisture content. Se-MB possessed the excellent whitening, moisturizing, and antioxidant efficacy, which could lay a scientific foundation for utilization and development of skincare products of Se-MB and its related industrial cosmetics products.
富硒绿豆（Se-MB）是绿豆（MB）与硒（Se）的结合物，具有多种潜在的生物活性。然而，关于富硒绿豆的护肤活性却知之甚少。对富硒绿豆发酵液（Se-MBFB）的化学成分进行了分析，以研究其美白、保湿和抗衰老活性。分析了其对酪氨酸酶的抑制作用、对黑素细胞（B16F10细胞）的抗黑素生成作用以及对人皮肤成纤维细胞（HDFs）的保湿作用。此外，还评估了富硒绿豆发酵液的自由基清除活性。为了验证富硒绿豆发酵液的效果及其实际应用潜力，对参与者进行了一项临床试验：31名年龄在25至60岁之间的中国女性，无色素沉着紊乱、无疾病、无过敏反应史且未在面部使用护肤品。参与者在洁面后使用富硒绿豆发酵液面膜15至20分钟。测量点为使用面膜后的第0周、第2周和第4周（W0、W2和W4），目标部位为脸颊和眼角。每次就诊时在目标部位记录以下参数：黑色素指数、肤色、角质层水分含量、经表皮水分流失和鱼尾纹。结果表明，富硒绿豆发酵液富含多酚、肽和γ-氨基丁酸（GABA），具有显著的自由基清除和酪氨酸酶抑制活性，减少了黑色素的合成，并上调了水通道蛋白3（AQP3）的表达。富硒绿豆发酵液面膜测试表明，使用富硒绿豆发酵液面膜4周后，参与者的面部变得更白，皱纹减少，水分含量增加。富硒绿豆具有优异的美白、保湿和抗氧化功效，可为富硒绿豆护肤品及其相关工业化妆品的开发利用奠定科学基础。
