微电子衍射：概念、实践与未来机遇。

Clabbers Max T B, Shiriaeva Anna, Gonen Tamir

Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.

Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.

IUCrJ. 2022 Jan 18;9(Pt 2):169-179. doi: 10.1107/S2052252521013063. eCollection 2022 Mar 1.

This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today's application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.

本文记录了在2021年于布拉格举行的国际晶体学联盟大会上发表的一场主题研讨会。文中描述了低温电子显微镜方法——微晶电子衍射，并将其置于大分子电子晶体学的背景下，从其起源于膜蛋白的二维晶体，到如今应用于尺寸仅为X射线晶体学所需晶体百万分之一大小的三维晶体。文中介绍了该方法开发和应用中的里程碑事件，并展望了未来。