Sagar Pankaj Kumar, Sharma Poonam, Singh Rambir
Department of Biotechnology, Bundelkhand University, Jhansi (U.P.), India.
Department of Zoology, Indira Gandhi National Tribal University (A Central University), Amarkantak, Madhya Pradesh, India.
J Pharmacopuncture. 2022 Mar 31;25(1):37-45. doi: 10.3831/KPI.2022.25.1.37.
The quorum-sensing-inhibitory and anti-biofilm activities of the methanol extract of leaves were determined against clinically isolated multidrug-resistant .
The preliminary anti-quorum-sensing (AQS) activity of eucalyptus was investigated against a biosensor strain ATCC 12472 (CV12472) by using the agar well diffusion method. The effect of sub-minimum inhibitory concentrations (sub-MICs) of the methanol extract of eucalyptus on different quorum-sensing-regulated virulence factors, such as swarming motility, pyocyanin pigment, exopolysaccharide (EPS), and biofilm formation, against clinical isolates (CIs 2, 3, and 4) and reference PA01 of were determined using the swarm diameter (mm)-measurement method, chloroform extraction method, phenol (5%)-sulphuric acid (concentrated) method, and the microtiter plate assay respectively, and the inhibition (%) in formation were calculated.
The preliminary AQS activity (violacein pigment inhibition) of eucalyptus was confirmed against ATCC 12472 (CV12472). The eucalyptus extract also showed concentration-dependent inhibition (%) of swarming motility, pyocyanin pigment, EPS, and biofilm formation in different CIs and PA01 of .
Our results revealed the effectiveness of the extract for the regulation of quorum-sensing-dependent virulence factors and biofilm formation at a reduced dose (sub-MICs) and suggest that may be a therapeutic agent for curing and controlling bacterial infection and thereby reducing the possibility of resistance development in pathogenic strains.
测定树叶甲醇提取物对临床分离的多重耐药菌的群体感应抑制和抗生物膜活性。
采用琼脂孔扩散法，研究桉树叶对生物传感器菌株ATCC 12472（CV12472）的初步抗群体感应（AQS）活性。分别采用群体直径（mm）测量法、氯仿萃取法、苯酚（5%）-硫酸（浓）法和微量滴定板法，测定桉树叶甲醇提取物的亚最小抑菌浓度（sub-MICs）对不同群体感应调节的毒力因子，如群集运动性、绿脓菌素色素、胞外多糖（EPS）和生物膜形成的影响，受试对象为临床分离株（CIs 2、3和4）和参照菌株PA01，并计算形成抑制率（%）。
证实桉树叶对ATCC 12472（CV12472）具有初步AQS活性（抑制紫色杆菌素色素）。桉树叶提取物对不同临床分离株和PA01中的群集运动性、绿脓菌素色素、EPS和生物膜形成也表现出浓度依赖性抑制（%）。
我们的结果揭示了桉树叶提取物在降低剂量（亚最小抑菌浓度）下调节群体感应依赖性毒力因子和生物膜形成的有效性，并表明桉树叶可能是一种治疗药物，用于治愈和控制细菌感染从而降低致病菌株产生耐药性的可能性。
