Sankar Ganesh P, Ravishankar Rai V
Department of Studies in Microbiology, University of Mysore, Mysore 570006, Karnataka, India.
J Tradit Complement Med. 2017 Jun 8;8(1):170-177. doi: 10.1016/j.jtcme.2017.05.008. eCollection 2018 Jan.
aeruginosa use small signaling molecules such as acyl homoserine lactones (AHLs), which play an important role in release virulence factors and toxin for further establishment of host infection. Thus, involving with the QS system would provide alternative ways of preventing the pathogenicity. In the present study, totally six medicinal plants (, , , , , ) were screened for anti-QS activity using biomonitor strain of CV12472. The primary screening of antimicrobial activity of all the plant extracts have inhibited the growth of tested bacterial species. Of these at the sub-minimum inhibitory concentration the methanol extract of (0.0625-0.5 mg/ml) has significantly inhibited violacein production (20.07-66.22%) in (CV12472). Consequently, the extract of has reduced the production of pyocyanin, exopolysaccharide and biofilm formation in strains. Fluorescence and scanning electron microscopy analysis confirmed the reduction of biofilm formation in strains when treated with . GC-MS analysis showed the active compounds inhibited the production of virulence factors of . The results suggest the possible use of this as an anti-QS and anti-biofilm agent to control infection. Interference of QS provides an important means for the inhibition of bacterial virulence and thus aids in treatment strategies.
铜绿假单胞菌利用诸如酰基高丝氨酸内酯（AHLs）等小信号分子，这些分子在释放毒力因子和毒素以进一步建立宿主感染方面发挥着重要作用。因此，参与群体感应系统将提供预防致病性的替代方法。在本研究中，使用CV12472生物监测菌株对总共六种药用植物（、、、、、）进行了抗群体感应活性筛选。所有植物提取物的抗菌活性初步筛选均抑制了受试细菌物种的生长。其中，在亚最小抑菌浓度下，（0.0625 - 0.5毫克/毫升）的甲醇提取物显著抑制了（CV12472）中紫菌素的产生（20.07 - 66.22%）。因此，提取物减少了铜绿假单胞菌菌株中绿脓菌素、胞外多糖的产生以及生物膜的形成。荧光和扫描电子显微镜分析证实，用处理后的铜绿假单胞菌菌株生物膜形成减少。气相色谱 - 质谱分析表明，活性化合物抑制了铜绿假单胞菌毒力因子的产生。结果表明，这种可能用作抗群体感应和抗生物膜剂来控制铜绿假单胞菌感染。群体感应的干扰为抑制细菌毒力提供了重要手段，从而有助于治疗策略。
