Attenuation of quorum-sensing-dependent virulence factors and biofilm formation by medicinal plants against antibiotic resistant .

aeruginosa use small signaling molecules such as acyl homoserine lactones (AHLs), which play an important role in release virulence factors and toxin for further establishment of host infection. Thus, involving with the QS system would provide alternative ways of preventing the pathogenicity. In the present study, totally six medicinal plants (, , , , , ) were screened for anti-QS activity using biomonitor strain of CV12472. The primary screening of antimicrobial activity of all the plant extracts have inhibited the growth of tested bacterial species. Of these at the sub-minimum inhibitory concentration the methanol extract of (0.0625-0.5 mg/ml) has significantly inhibited violacein production (20.07-66.22%) in (CV12472). Consequently, the extract of has reduced the production of pyocyanin, exopolysaccharide and biofilm formation in strains. Fluorescence and scanning electron microscopy analysis confirmed the reduction of biofilm formation in strains when treated with . GC-MS analysis showed the active compounds inhibited the production of virulence factors of . The results suggest the possible use of this as an anti-QS and anti-biofilm agent to control infection. Interference of QS provides an important means for the inhibition of bacterial virulence and thus aids in treatment strategies.