核糖核蛋白的电泳揭示了酵母剪接复合体的有序组装途径。
Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
作者信息
Pikielny C W, Rymond B C, Rosbash M
出版信息
Nature. 1986;324(6095):341-5. doi: 10.1038/324341a0.
DOI:10.1038/324341a0
PMID:3537805
Abstract
Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.
摘要
在体外剪接过程中，与酵母前体信使RNA形成的三种剪接复合体，在非特异性竞争RNA存在的情况下孵育后，可通过非变性凝胶电泳进行分离。这些复合体出现的时间进程及其组成表明，它们代表了剪接复合体组装的有序途径。
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