• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

空间CITE-seq：空间分辨的高多重蛋白质和全转录组共映射

Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.

作者信息

Liu Yang, DiStasio Marcello, Su Graham, Asashima Hiromitsu, Enninful Archibald, Qin Xiaoyu, Deng Yanxiang, Bordignon Pino, Cassano Marco, Tomayko Mary, Xu Mina, Halene Stephanie, Craft Joseph E, Hafler David, Fan Rong

机构信息

Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.

Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT 06520, USA.

出版信息

Res Sq. 2022 Mar 30:rs.3.rs-1499315. doi: 10.21203/rs.3.rs-1499315/v1.

DOI:10.21203/rs.3.rs-1499315/v1
PMID:35378748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8978952/
Abstract

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

摘要

我们展示了用于高多重蛋白质和全转录组共映射的空间CITE-seq技术，该技术首次用于分析多种小鼠组织类型中的198种蛋白质和转录组。随后，它被应用于人体组织，以测量283种蛋白质和转录组，揭示了扁桃体中空间上不同的生发中心反应以及COVID-19 mRNA疫苗注射部位皮肤中的早期免疫激活。空间CITE-seq可能在生物医学研究中找到一系列应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/8ca72f43d206/nihpp-rs1499315v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/99da992630f2/nihpp-rs1499315v1-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/6bbf7acb7a22/nihpp-rs1499315v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/8ca72f43d206/nihpp-rs1499315v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/99da992630f2/nihpp-rs1499315v1-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/6bbf7acb7a22/nihpp-rs1499315v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3c9/8978952/8ca72f43d206/nihpp-rs1499315v1-f0003.jpg

相似文献

1
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.空间CITE-seq：空间分辨的高多重蛋白质和全转录组共映射
Res Sq. 2022 Mar 30:rs.3.rs-1499315. doi: 10.21203/rs.3.rs-1499315/v1.
2
High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq.高多重蛋白和全转录组共定位与空间 CITE-seq 在细胞分辨率下。
Nat Biotechnol. 2023 Oct;41(10):1405-1409. doi: 10.1038/s41587-023-01676-0. Epub 2023 Feb 23.
3
Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling.利用数字空间分析技术在人体和小鼠组织中进行空间分辨的全转录组分析。
Genome Res. 2022 Oct;32(10):1892-1905. doi: 10.1101/gr.276206.121. Epub 2022 Sep 13.
4
Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles.超高多重空间蛋白质基因组学研究揭示了巨细胞胶质母细胞瘤多形性免疫浸润的独特蛋白和 RNA 表达谱。
Cancer Res Commun. 2023 May 3;3(5):763-779. doi: 10.1158/2767-9764.CRC-22-0396. eCollection 2023 May.
5
High-Plex Spatially Resolved RNA and Protein Detection Using Digital Spatial Profiling: A Technology Designed for Immuno-oncology Biomarker Discovery and Translational Research.使用数字空间分析进行高维多空间分辨RNA和蛋白质检测：一种专为免疫肿瘤学生物标志物发现和转化研究设计的技术。
Methods Mol Biol. 2020;2055:563-583. doi: 10.1007/978-1-4939-9773-2_25.
6
Spatial profiling of chromatin accessibility in mouse and human tissues.在小鼠和人体组织中进行染色质可及性的空间分析。
Nature. 2022 Sep;609(7926):375-383. doi: 10.1038/s41586-022-05094-1. Epub 2022 Aug 17.
7
Imputation of spatially-resolved transcriptomes by graph-regularized tensor completion.基于图正则化张量补全的空间分辨转录组推断。
PLoS Comput Biol. 2021 Apr 7;17(4):e1008218. doi: 10.1371/journal.pcbi.1008218. eCollection 2021 Apr.
8
A molecularly defined and spatially resolved cell atlas of the whole mouse brain.一份分子定义且空间解析的全小鼠脑图谱。
bioRxiv. 2023 Mar 7:2023.03.06.531348. doi: 10.1101/2023.03.06.531348.
9
Spatial-ID: a cell typing method for spatially resolved transcriptomics via transfer learning and spatial embedding.空间 ID：一种通过迁移学习和空间嵌入进行空间分辨转录组学的细胞分型方法。
Nat Commun. 2022 Dec 10;13(1):7640. doi: 10.1038/s41467-022-35288-0.
10
Characterizing spatial gene expression heterogeneity in spatially resolved single-cell transcriptomic data with nonuniform cellular densities.刻画具有非均匀细胞密度的空间分辨单细胞转录组数据中空间基因表达异质性。
Genome Res. 2021 Oct;31(10):1843-1855. doi: 10.1101/gr.271288.120. Epub 2021 May 25.

引用本文的文献

1
Methods and applications for single-cell and spatial multi-omics.单细胞和空间多组学的方法和应用。
Nat Rev Genet. 2023 Aug;24(8):494-515. doi: 10.1038/s41576-023-00580-2. Epub 2023 Mar 2.

本文引用的文献

1
SM-Omics is an automated platform for high-throughput spatial multi-omics.SM-Omics 是一个自动化的高通量空间多组学平台。
Nat Commun. 2022 Feb 10;13(1):795. doi: 10.1038/s41467-022-28445-y.
2
Three-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment.用于组织和肿瘤微环境的高度多重分子和细胞图谱分析的三维成像质谱流式细胞术。
Nat Cancer. 2022 Jan;3(1):122-133. doi: 10.1038/s43018-021-00301-w. Epub 2021 Dec 24.
3
Committed Human CD23-Negative Light-Zone Germinal Center B Cells Delineate Transcriptional Program Supporting Plasma Cell Differentiation.
滤泡中心 B 细胞 committed Human CD23-negative Light-Zone 可划分支持浆细胞分化的转录程序。
Front Immunol. 2021 Dec 2;12:744573. doi: 10.3389/fimmu.2021.744573. eCollection 2021.
4
Spatial multi-omics sequencing for fixed tissue via DBiT-seq.基于 DBiT-seq 的固定组织空间多组学测序。
STAR Protoc. 2021 May 11;2(2):100532. doi: 10.1016/j.xpro.2021.100532. eCollection 2021 Jun 18.
5
Spatially resolved transcriptomics adds a new dimension to genomics.空间分辨转录组学为基因组学增添了新的维度。
Nat Methods. 2021 Jan;18(1):15-18. doi: 10.1038/s41592-020-01038-7.
6
High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue.通过组织中的确定性条形码进行高空间分辨率多组学测序。
Cell. 2020 Dec 10;183(6):1665-1681.e18. doi: 10.1016/j.cell.2020.10.026. Epub 2020 Nov 13.
7
Shared and distinct roles of T peripheral helper and T follicular helper cells in human diseases.辅助性 T 细胞（Tfh）和滤泡辅助性 T 细胞在人类疾病中的共享和独特作用。
Cell Mol Immunol. 2021 Mar;18(3):523-527. doi: 10.1038/s41423-020-00529-z. Epub 2020 Aug 31.
8
Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections.微流控辅助多重生物标志物检测用于肿瘤切片中免疫细胞的原位图谱绘制。
Microsyst Nanoeng. 2019 Nov 6;5:59. doi: 10.1038/s41378-019-0104-z. eCollection 2019.
9
Comprehensive Integration of Single-Cell Data.单细胞数据的综合整合。
Cell. 2019 Jun 13;177(7):1888-1902.e21. doi: 10.1016/j.cell.2019.05.031. Epub 2019 Jun 6.
10
Spatial transcriptomics coming of age.空间转录组学走向成熟。
Nat Rev Genet. 2019 Jun;20(6):317. doi: 10.1038/s41576-019-0129-z.