Csako G, Tsai C M, Hochstein H D, Elin R J
Radiat Res. 1986 Nov;108(2):158-66.
Increasing concentrations of a highly purified bacterial lipopolysaccharide preparation, the U.S. Reference Standard Endotoxin, were exposed to increasing doses of ionizing radiation from a 60Co source. At identical radiation doses both the structural change and Limulus amebocyte lysate (LAL) reactivity were progressively smaller with increasing concentrations of the lipopolysaccharide in an aqueous medium. Under the experimental conditions used, there was a linear relationship between the endotoxin concentration and radiation dose for the structural changes. In contrast to endotoxin in aqueous medium, endotoxin irradiated in its dry state showed no decrease in LAL reactivity and rabbit pyrogenicity. Endotoxin exposed to radiation in water in the presence of albumin showed a much smaller decrease in LAL and pyrogenic activities than expected. The results show that the concentration, physical state, and purity of endotoxin influence its structural and functional alteration by ionizing radiation.
将高纯度细菌脂多糖制剂（美国参考标准内毒素）的浓度不断增加，使其暴露于来自60Co源的不断增加剂量的电离辐射下。在相同的辐射剂量下，随着水性介质中脂多糖浓度的增加，结构变化和鲎试剂（LAL）反应性都逐渐变小。在所使用的实验条件下，内毒素浓度与结构变化的辐射剂量之间存在线性关系。与水性介质中的内毒素不同，干燥状态下辐照的内毒素LAL反应性和兔致热原性没有降低。在白蛋白存在下于水中暴露于辐射的内毒素，其LAL和致热活性的降低幅度比预期小得多。结果表明，内毒素的浓度、物理状态和纯度会影响其因电离辐射而发生的结构和功能改变。
