Infectious Diseases Translational Research Programme, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117599, Singapore.
Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore.
Sci Transl Med. 2022 Apr 6;14(639):eabj4124. doi: 10.1126/scitranslmed.abj4124.
Rapid diagnosis is one key pillar to end tuberculosis (TB). Point-of-care tests (POCTs) facilitate early detection, immediate treatment, and reduced transmission of TB disease. This Review evaluates current diagnostic assays endorsed by the World Health Organization and identifies the gaps between existing conventional tests and the ideal POCT. We discuss the commercial development of new rapid tests and research studies on nonsputum-based diagnostic biomarkers from both pathogen and host. Last, we highlight advances in integrated microfluidics technology that may aid the development of new POCTs.
快速诊断是终结结核病（TB）的关键支柱之一。即时检测（POCT）有助于早期发现、立即治疗和减少结核病的传播。本综述评估了世界卫生组织认可的当前诊断检测方法，并确定了现有常规检测方法与理想 POCT 之间的差距。我们讨论了新的快速检测方法的商业开发以及基于病原体和宿主的非痰液诊断生物标志物的研究进展。最后，我们强调了集成微流控技术的进步，这可能有助于新 POCT 的开发。
