Yang Fan, Deng Xuan, Yu Ye, Luo Lei, Chen Xianda, Zheng Jinping, Qiu Yugang, Xiao Feng, Xie Xiaomei, Zhao Yuzheng, Guo Jun, Hu Feifei, Zhang Xuguang, Ju Zhenyu, Zhou Yong
The First Affiliated Hospital of Jinan University, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China.
Clinical Research Institute, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Endocrinol (Lausanne). 2022 Mar 21;13:829658. doi: 10.3389/fendo.2022.829658. eCollection 2022.
NAD, nicotinamide adenine dinucleotide, is mostly described to associate with the aging process. We aimed to investigate the association between human whole blood NAD contents and aging in a relative large-scale community-based population and further to address the gender impact on this association.
We recruited 1,518 participants aged over 18 years old and free of cardiovascular and any type of cancer from the Jidong community from 2019 to 2020. Whole blood NAD level was measured by cycling assay and LC-mass spectroscopy assay. The chronological age and clinical data were collected using standard questionnaires. The participants were divided into five groups according to their chronological age. General liner regression model was performed to analyze the association between NAD contents and aging. In addition, we also conducted subgroup analysis by gender.
The mean age of included 1,518 participants was 43.0 years, and 52.6% of them were men. The average levels of whole blood NAD of total participants was 33.0 ± 5.5 μmol/L. The whole blood NAD contents in men were significantly higher than that in women (34.5 vs. 31.3 μmol/L). There was significant difference in the meat diet among NAD quartile groups (p = 0.01). We observed a decline trend of NAD contents with aging before 50 years in total participants with significant level in 40-49 years old group (β coefficients with 95% confidence interval (95% CI): -1.12 (-2.18, -0.06)), while this trend disappeared after the 50 years. In addition, this association was significantly altered by gender (p for interaction = 0.003). In men, as compared with ≤29 years group, adjusted β coefficient decreased with aging but was only significant in the ≥60 year group (β,-2.16; 95% CI, -4.16 to -0.15). In females, the level of whole blood NAD did not significantly differ among five age groups and without the trend as males.
Association of whole blood NAD contents with aging significantly differed in males and females. The loss of blood NAD with aging only was observed in males, especially in the male middle-aged population. It is crucial to consider the gender difference in further NAD related studies in the future.
烟酰胺腺嘌呤二核苷酸（NAD）大多被描述为与衰老过程相关。我们旨在调查在一个相对大规模的社区人群中，人类全血NAD含量与衰老之间的关联，并进一步探讨性别对这种关联的影响。
我们在2019年至2020年期间从冀东社区招募了1518名年龄超过18岁且无心血管疾病和任何类型癌症的参与者。通过循环分析和液相色谱-质谱分析法测量全血NAD水平。使用标准问卷收集参与者的实际年龄和临床数据。根据实际年龄将参与者分为五组。采用一般线性回归模型分析NAD含量与衰老之间的关联。此外，我们还按性别进行了亚组分析。
纳入的1518名参与者的平均年龄为43.0岁，其中52.6%为男性。所有参与者全血NAD的平均水平为33.0±5.5μmol/L。男性的全血NAD含量显著高于女性（34.5对31.3μmol/L）。NAD四分位数组之间的肉类饮食存在显著差异（p = 0.01）。我们观察到，在所有参与者中，50岁之前NAD含量随衰老呈下降趋势，在40 - 49岁组具有显著水平（β系数及95%置信区间（95%CI）：-1.12（-2.18，-0.06）），而50岁之后这种趋势消失。此外，这种关联因性别而有显著改变（交互作用p = 0.003）。在男性中，与≤29岁组相比，调整后的β系数随衰老而降低，但仅在≥60岁组显著（β，-2.16；95%CI，-4.16至-0.15）。在女性中，五个年龄组的全血NAD水平无显著差异，且没有男性那样的趋势。
全血NAD含量与衰老之间的关联在男性和女性中存在显著差异。仅在男性中观察到血液NAD随衰老而减少，尤其是在男性中年人群中。在未来进一步的NAD相关研究中考虑性别差异至关重要。
