The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral and Maxillofacial-Head and Neck Oncology, School of Stomatology-Hospital of Stomatology, Wuhan University, Wuhan, China.
Oral Dis. 2023 Jul;29(5):1967-1978. doi: 10.1111/odi.14203. Epub 2022 Apr 19.
Tumor-stroma ratio (TSR) is a promising parameter representing the abundance of the stroma which has been validated in many solid tumors. However, it is still not clear which part of stroma mainly contribute to the prognostic value of TSR. The aim of this study is to confirm the prognostic value of TSR in a large cohort of oral squamous cell carcinoma (OSCC) and further demonstrated that cancer-associated fibroblasts (CAFs)-stroma ratio (CSR) contributed to the prognostic value of TSR.
TSR was evaluated on hematoxylin and eosin stained tissue samples from 581 patients with OSCC, which divides patients into high (>50%) and low (<50%) stroma. Then, CSR was estimated on immunohistochemical staining slides of 100 patients selected from 581 patients.
In multivariate analysis, TSR was identified as an independent prognostic factor for disease-free survival (DFS) (p < 0.001) and oral cancer-specific survival (OCSS) (p < 0.001). The interaction term reached statistical significance for histological grade for DFS and OCSS separately. Furthermore, the high-stroma group had a higher CSR than the low-stroma group.
The prognostic value of TSR is validated in OSCC particularly in moderate and high differentiation, and CSR plays its part in the prognosis of TSR.
肿瘤基质比（TSR）是一种有前途的参数，代表基质的丰度，已在许多实体瘤中得到验证。然而，目前尚不清楚基质的哪一部分主要对 TSR 的预后价值有贡献。本研究旨在确认 TSR 在大型口腔鳞状细胞癌（OSCC）队列中的预后价值，并进一步证明癌相关成纤维细胞（CAFs）-基质比（CSR）对 TSR 的预后价值有贡献。
在 581 例 OSCC 患者的苏木精和伊红染色组织样本上评估 TSR，将患者分为高（>50%）和低（<50%）基质。然后，从 581 例患者中选择 100 例患者的免疫组织化学染色切片上评估 CSR。
在多变量分析中，TSR 被确定为无病生存（DFS）（p<0.001）和口腔癌特异性生存（OCSS）（p<0.001）的独立预后因素。DFS 和 OCSS 分别在组织学分级方面，交互项达到统计学意义。此外，高基质组的 CSR 高于低基质组。
TSR 的预后价值在 OSCC 中得到验证，特别是在中高分化的情况下，CSR 在 TSR 的预后中发挥了作用。
