Barati Shoorche Amin, Mohammadkarim Alireza, Jadidi Majid, Bahraminasab Marjan
Department of Medical Physics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Lasers Med Sci. 2022 Sep;37(7):2855-2863. doi: 10.1007/s10103-022-03554-8. Epub 2022 Apr 8.
Photobiomodulation (PBM) therapy utilizes low-power lasers to modulate the viability of living human cells and leads to changes in proliferation, differentiation, adhesion and gene expression, even though the rearrangement of cytoskeleton was not previously studied. The present study aims to evaluate the photobiological effects on the elastic behavior of human osteosarcoma cells (MG-63) and their morphological changes. Fluorescence staining, confocal imaging and atomic force microscopy (AFM) topography were performed to study the effects of PBM therapy with the exposure of 532 nm-25mW, 650 nm-3mW, 650 nm-150mW and 780 nm-70mW beams following the 5-min continuous irradiation. The area of each beam was 3.14cm with a source-surface distance of 20 cm. Besides the cell proliferation assessment, the migratory potential of MG-63 was determined with the wound healing technique. The results indicated an increase in stiffness and shape index of radiation-induced cells 24 h after exposure along with the obvious F-actins changes. But, cell stiffening was not observed 72 h after 532 nm laser irradiation. Also, a decrease in the migration rate was seen in all of the groups after 72 h of irradiation except cells treated with 532 nm wavelength. However, 532 nm laser beams increase the migratory potential 24 h after exposure. Within 72 h after irradiation, the cell proliferation was only affected by applying 532 nm and 650 nm-150mW laser beams. It was concluded that applying photobiomodulation with wavelengths of 650 nm (at both utilized powers) and 780 nm alters the migration capability and provides a quantitative description of cytoskeletal changes. Moreover, membrane stiffening can be considered as the biological marker of PBM treatments.
光生物调节（PBM）疗法利用低功率激光调节人体活细胞的活力，并导致细胞增殖、分化、黏附和基因表达的变化，尽管此前尚未研究细胞骨架的重排。本研究旨在评估光生物对人骨肉瘤细胞（MG-63）弹性行为及其形态变化的影响。采用荧光染色、共聚焦成像和原子力显微镜（AFM）形貌分析，研究了532 nm-25 mW、650 nm-3 mW、650 nm-150 mW和780 nm-70 mW光束连续照射5分钟后PBM疗法的效果。每束光的面积为3.14平方厘米，源表面距离为20厘米。除了细胞增殖评估外，还采用伤口愈合技术测定了MG-63的迁移潜力。结果表明，照射后24小时，辐射诱导细胞的硬度和形状指数增加，同时F-肌动蛋白明显变化。但是，532 nm激光照射72小时后未观察到细胞变硬。此外，照射72小时后，除532 nm波长处理的细胞外，所有组的迁移率均下降。然而，532 nm激光束在照射后24小时增加了迁移潜力。照射后72小时内，仅532 nm和650 nm-150 mW激光束影响细胞增殖。得出的结论是，应用650 nm（两种功率）和780 nm波长的光生物调节可改变迁移能力，并对细胞骨架变化进行定量描述。此外，膜硬度可被视为PBM治疗的生物学标志物。
