呼吸道合胞病毒血清IgM抗体捕获酶联免疫吸附测定
Capture-ELISA for serum IgM antibody to respiratory syncytial virus.
作者信息
Cevenini R, Donati M, Bertini S, Moroni A, Sambri V
出版信息
J Hyg (Lond). 1986 Dec;97(3):511-7. doi: 10.1017/s0022172400063713.
A four-component solid-phase capture enzyme immunoassay was set up to test for serum IgM antibody to respiratory syncytial (RS) virus and was compared with immunofluorescence assay (IFA). A total of 128 young children with acute respiratory infections were studied. Thirty-six were shown to be RS virus-positive by the detection of RS virus in nasopharyngeal secretions and 92 were RS virus-negative. A serum specimen was collected after admission to the hospital (days 0-4) and a further specimen was obtained during days 10-14. Out of 36 RS virus-positive patients, 28 (77.7%) were found to be positive for IgM by both capture-ELISA and IFA. Out of 92 RS virus-negative patients 5 (5.4%) were IgM-positive. Four false-positive results were obtained by IFA due to the presence of rheumatoid factor. The capture-ELISA was shown to be a reliable technique in detecting specific IgM antibody to RS virus.
建立了一种四组分固相捕获酶免疫测定法来检测血清中呼吸道合胞（RS）病毒的IgM抗体，并与免疫荧光测定法（IFA）进行比较。共研究了128例急性呼吸道感染的幼儿。通过检测鼻咽分泌物中的RS病毒，36例显示为RS病毒阳性，92例为RS病毒阴性。入院后（第0 - 4天）采集血清标本，并在第10 - 14天获取另一标本。在36例RS病毒阳性患者中，28例（77.7%）通过捕获酶联免疫吸附测定法和IFA检测均为IgM阳性。在92例RS病毒阴性患者中，5例（5.4%）为IgM阳性。由于存在类风湿因子，IFA获得了4例假阳性结果。捕获酶联免疫吸附测定法被证明是检测RS病毒特异性IgM抗体的可靠技术。
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