Hornsleth A, Friis B, Andersen P, Brenøe E
J Med Virol. 1982;10(4):273-81. doi: 10.1002/jmv.1890100407.
Samples of nasopharyngeal secretions (NPS) obtained from 140 infants and children with acute respiratory disease were examined for the presence of respiratory syncytial (RS) virus by ELISA. An antiserum produced in rabbits against RS-virus polypeptides was used both as the "capture" antibody and as conjugate in a double-antibody sandwich technique. RS virus was detected by ELISA in specimens of NPS obtained from 72 (51%) of the patients. By comparison RS virus was shown to be present in 86 (61%) of the patients when the immunofluorescent antibody (IF) technique was employed. Samples obtained from 78 of the 140 patients were also examined by inoculation into tissue cultures, and RS-virus was isolated from 47 patients. RS virus was detected by ELISA in 37 (79%) and by IF technique in 43 (91%) of these 47 patients. The IF technique was found to be more sensitive than ELISA, especially in patients older than 9 months and for the examination of specimens of NPS containing only a small percentage of RS-virus antigen-positive cells.
采用酶联免疫吸附测定（ELISA）法，对140例患急性呼吸道疾病的婴幼儿和儿童的鼻咽分泌物（NPS）样本进行检测，以确定其中是否存在呼吸道合胞（RS）病毒。在双抗体夹心技术中，用兔抗RS病毒多肽产生的抗血清作为“捕获”抗体和共轭物。通过ELISA法在72例（51%）患者的NPS样本中检测到RS病毒。相比之下，采用免疫荧光抗体（IF）技术时，86例（61%）患者体内显示存在RS病毒。对140例患者中的78例采集的样本还通过接种到组织培养物中进行检测，从47例患者中分离出RS病毒。在这47例患者中，ELISA法在37例（79%）中检测到RS病毒，IF技术在43例（91%）中检测到RS病毒。发现IF技术比ELISA法更灵敏，尤其是对于9个月以上的患者以及检测仅含有小百分比RS病毒抗原阳性细胞的NPS样本。
