Laboratory for Emerging Infectious Diseases, University of Buea, PO Box 63, Buea, Cameroon.
Department of Microbiology and Parasitology, University of Buea, PO Box 63, Buea, Cameroon.
Biomed Res Int. 2022 Mar 31;2022:9977371. doi: 10.1155/2022/9977371. eCollection 2022.
The release of untreated wastes from abattoirs into the environment and nearby water bodies poses a significant threat to public health. Such litters may contain pathogens, including antibiotic-resistant bacteria. This study investigated 80 samples collected from butchering tables, slaughter slabs, meat rinsing points, and abattoir wastes receiving water from two abattoirs (A and B). Total count (TEC) for each sample was determined, and (. ), spp., spp., and () were isolated and identified. Antimicrobial susceptibility testing was done on all bacterial isolates against nine locally used antibiotics. Overall, 118 bacterial isolates, comprising (42.5%), spp. (27.5%), spp. (37.5%), and (40.0%), were recovered. Of the 118 bacterial isolates, 104 (88.1%) were multidrug-resistant, including 58 (55.8%) from abattoir A and 46 (44.2%) from abattoir B; however, this difference was not statistically significant ( = 0.6837). Of the 32 . isolates, 29 (90.6%) were multidrug-resistant. All . were 100% sensitive to vancomycin, kanamycin, and amikacin. Similarly, 31 (91.2%) of the 34 . isolates recovered in this study were multidrug-resistant. spp. and spp. also showed high levels of multidrug resistance corresponding to 81.8% and 86.7%, respectively. All isolates of , , and were 100% resistant to ampicillin and 100% sensitive to ciprofloxacin. Minimum and maximum mean values for TEC were 3.62-5.83 log CFU/mL for abattoir A and 4.08-5.56 log CFU/mL for abattoir B. The highest and lowest TEC counts were from slaughter slab and upstream water, respectively, in each abattoir. Our results indicate a predominance of multidrug-resistant bacteria in abattoir wastes and their receiving waters in the study sites. Hence, we recommend the treatment of abattoir wastes before disposal and improved hygiene and sanitation practices to enhance public health.
屠宰场未经处理的废物排放到环境和附近的水体中，对公共健康构成重大威胁。这些废物可能含有病原体，包括抗生素耐药菌。本研究调查了来自两个屠宰场（A 和 B）的屠宰台、屠宰板、肉类冲洗点和屠宰废物接收水的 80 个样本。每个样本的总计数（TEC）均已确定，并分离和鉴定了（ ）、 spp.、 spp. 和 （ ）。对所有细菌分离株进行了针对 9 种当地使用抗生素的药敏试验。总体而言，从屠宰场 A 回收了 118 株细菌分离株，包括（42.5%）、 spp.（27.5%）、 spp.（37.5%）和 （40.0%），从屠宰场 B 回收了 118 株细菌分离株（55.8%）和 46 株（44.2%）。118 株细菌分离株中，有 104 株（88.1%）为多药耐药株，包括屠宰场 A 的 58 株（55.8%）和屠宰场 B 的 46 株（44.2%）；然而，这一差异无统计学意义（=0.6837）。32 株 中，有 29 株（90.6%）为多药耐药株。所有. 对万古霉素、卡那霉素和阿米卡星 100%敏感。同样，在本研究中回收的 34 株. 分离株中，有 31 株（91.2%）为多药耐药株。 spp. 和 spp. 也表现出高水平的多药耐药性，分别为 81.8%和 86.7%。所有 、 和 的分离株对氨苄西林 100%耐药，对环丙沙星 100%敏感。屠宰场 A 的 TEC 最小值和最大值分别为 3.62-5.83 log CFU/mL，屠宰场 B 的 TEC 最小值和最大值分别为 4.08-5.56 log CFU/mL。每个屠宰场中，来自屠宰台和上游水的 TEC 计数最高和最低。我们的结果表明，研究地点的屠宰场废物及其接收水中存在大量多药耐药菌。因此，我们建议在处理之前对屠宰场废物进行处理，并改善卫生和环境卫生措施，以增强公共卫生。
