State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
ACS Appl Mater Interfaces. 2022 Apr 20;14(15):17142-17152. doi: 10.1021/acsami.2c02503. Epub 2022 Apr 11.
Skin wound infection caused by methicillin-resistant (MRSA) is an urgent concern. Photodynamic therapy has emerged as a promising means of combating bacterial infection. However, continuous or repeated in situ light excitation is required for photosensitizers to produce reactive oxygen species (ROS), and most photosensitizers need sufficient oxygen to produce singlet oxygen (O), which greatly limits their clinical application. In this work, we report the preparation of ZnGeO:Cu (ZGC) persistent luminescence nanorods with excellent ability for persistent ROS production after stopping excitation for MRSA infectious wound healing. The prepared ZGC nanorods were loaded into dissolvable microneedles (MNs) (ZGC@MNs) to penetrate biofilms and treat MRSA-infected wounds in a minimally invasive manner. ZGC showed a long-persistent photocatalytic effect to constantly produce multiple ROS (O, hydroxyl radical, and superoxide radical) accompanied by persistent luminescence after a pre-illumination. The MN tips of ZGC@MNs were rapidly dissolved to release ZGC for the continuous production of multiple ROS for at least 48 h with no need for in situ excitation and no special requirement on the amount of oxygen for eliminating MRSA biofilms. The developed ZGC@MN patches exhibited excellent antibacterial activity and biocompatibility for effectively reducing inflammation and promoting wound healing in vivo.
耐甲氧西林金黄色葡萄球菌（MRSA）引起的皮肤伤口感染令人担忧。光动力疗法已成为一种有前途的对抗细菌感染的方法。然而，大多数光动力疗法需要足够的氧气才能产生单线态氧（O），因此需要持续或重复的原位光激发来产生活性氧（ROS），这极大地限制了它们的临床应用。在这项工作中，我们报告了一种制备 ZnGeO：Cu（ZGC）持久发光纳米棒的方法，该纳米棒在停止激发后具有出色的持续产生 ROS 的能力，可用于治疗耐甲氧西林金黄色葡萄球菌感染性伤口愈合。制备的 ZGC 纳米棒被装载到可溶解的微针（MN）中（ZGC@MNs），以微创的方式穿透生物膜并治疗耐甲氧西林金黄色葡萄球菌感染的伤口。ZGC 在预照射后表现出长持续的光催化效应，持续产生多种 ROS（O、羟基自由基和超氧自由基）并伴有持久发光。ZGC@MNs 的 MN 尖端迅速溶解，以至少 48 小时持续释放 ZGC，从而持续产生多种 ROS，无需原位激发，也不需要特殊的氧气量来消除耐甲氧西林金黄色葡萄球菌生物膜。开发的 ZGC@MN 贴片表现出出色的抗菌活性和生物相容性，可有效减轻炎症并促进体内伤口愈合。
