Neuroscience, Trauma and Ophthalmology, Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK.
NIHR Surgical Reconstruction and Microbiology Research Centre, University Hospitals Birmingham, Birmingham B15 2TH, UK.
Cells. 2022 Apr 5;11(7):1227. doi: 10.3390/cells11071227.
Traumatic brain injury (TBI) is a significant global health problem, for which no disease-modifying therapeutics are currently available to improve survival and outcomes. Current neuromonitoring modalities are unable to reflect the complex and changing pathophysiological processes of the acute changes that occur after TBI. Raman spectroscopy (RS) is a powerful, label-free, optical tool which can provide detailed biochemical data in vivo. A systematic review of the literature is presented of available evidence for the use of RS in TBI. Seven research studies met the inclusion/exclusion criteria with all studies being performed in pre-clinical models. None of the studies reported the in vivo application of RS, with spectral acquisition performed ex vivo and one performed in vitro. Four further studies were included that related to the use of RS in analogous brain injury models, and a further five utilised RS in ex vivo biofluid studies for diagnosis or monitoring of TBI. RS is identified as a potential means to identify injury severity and metabolic dysfunction which may hold translational value. In relation to the available evidence, the translational potentials and barriers are discussed. This systematic review supports the further translational development of RS in TBI to fully ascertain its potential for enhancing patient care.
创伤性脑损伤（TBI）是一个重大的全球健康问题，目前尚无任何疾病修饰疗法可用于改善生存和预后。目前的神经监测方式无法反映 TBI 后发生的急性变化的复杂和不断变化的病理生理过程。拉曼光谱（RS）是一种强大的、无标记的光学工具，可提供体内详细的生化数据。本文对 RS 在 TBI 中的应用进行了系统的文献回顾。有 7 项研究符合纳入/排除标准，且均在临床前模型中进行。没有一项研究报告了 RS 的体内应用，光谱采集是在离体和体外进行的。另外还有 4 项研究涉及 RS 在类似脑损伤模型中的应用，另有 5 项研究将 RS 用于体外生物流体研究以诊断或监测 TBI。RS 被认为是一种识别损伤严重程度和代谢功能障碍的潜在手段，可能具有转化价值。根据现有证据，讨论了转化的潜力和障碍。本系统评价支持进一步将 RS 转化应用于 TBI，以充分确定其在改善患者护理方面的潜力。
