Falak Shahkar, Shin Bo Kyoung, Huh Do Sung
Department of Chemistry, Nano Science and Engineering, Center of Nano Manufacturing, Inje University, Gimhae-si 50834, Korea.
Nanomaterials (Basel). 2022 Mar 25;12(7):1085. doi: 10.3390/nano12071085.
We have fabricated poly(ε-caprolactone) (PCL) films with flat and honeycomb-patterned (HCP) structures to coat polyaniline (PANI) on the film surface. In addition, the effect of chemical modification of PANI by sulfuric acid (HSO) was also studied for antibacterial activity. The flat and HCP PCL films were obtained by simple evaporation of the solvent and via the breath figure (BF) method, respectively. The morphology and chemical composition of PANI coated on the film surface were evaluated by scanning electron microscopy (SEM) and X-ray spectroscopy (EDX). Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analyses (TGA) were obtained to identify the PANI coating. The wettability and conductivity of the films were also measured. Applicational aspects were evaluated by assessing antibacterial and antibiofilm activity against () and (). The EDX, TGA, and FT-IR findings indicated chemical modification of PCL film by PANI and HSO. The conductivity of the films was increased by the coating of PANI to the patterned surface and additionally increased by the chemically modified PANI. The antibacterial activity was 69.79%, 78.27%, and 88% against , and 32.73%, 62.65%, and 87.97% against , for flat PANI, HCP PANI, and HSO-treated HCP films, respectively. Likewise, the PANI coated flat, HCP, and HSO-treated HCP films inhibited biofilm formation by around 41.62%, 63%, and 83.88% and biofilm formation by 17.81%, 69.83%, and 96.57%, respectively. The antibacterial activity of the HCP film was higher than that of flat PANI films, probably due to the higher coating of PANI on the HCP surface. Moreover, sulfonation of the HCP film with HSO might have improved the wettability, thereby enhancing the antibacterial and antibiofilm properties. Our results showed that topographical changes, as well as doping, offer simple and cost-effective ways to modify the structural and functional properties of films.
我们制备了具有平整结构和蜂窝状图案（HCP）结构的聚（ε-己内酯）（PCL）薄膜，用于在薄膜表面包覆聚苯胺（PANI）。此外，还研究了用硫酸（HSO）对聚苯胺进行化学改性对抗菌活性的影响。平整的PCL薄膜和HCP PCL薄膜分别通过简单蒸发溶剂和呼吸图案（BF）法获得。通过扫描电子显微镜（SEM）和X射线光谱（EDX）对包覆在薄膜表面的聚苯胺的形态和化学成分进行了评估。利用傅里叶变换红外光谱（FT-IR）和热重分析（TGA）来鉴定聚苯胺涂层。还测量了薄膜的润湿性和导电性。通过评估对（）和（）的抗菌和抗生物膜活性来评价其应用方面。EDX、TGA和FT-IR的结果表明聚苯胺和HSO对PCL薄膜进行了化学改性。通过在图案化表面包覆聚苯胺，薄膜的导电性增加，并且通过化学改性的聚苯胺进一步增加。对于平整聚苯胺薄膜、HCP聚苯胺薄膜和经HSO处理的HCP薄膜，其对的抗菌活性分别为69.79%、78.27%和88%，对的抗菌活性分别为32.73%、62.65%和87.97%。同样，包覆有聚苯胺的平整薄膜、HCP薄膜和经HSO处理的HCP薄膜分别抑制生物膜形成约41.62%、63%和83.88%，以及抑制生物膜形成17.81%、69.83%和96.57%。HCP薄膜的抗菌活性高于平整聚苯胺薄膜，这可能是由于聚苯胺在HCP表面的包覆量更高。此外，用HSO对HCP薄膜进行磺化可能改善了润湿性，从而增强了抗菌和抗生物膜性能。我们的结果表明，形貌变化以及掺杂为改变薄膜的结构和功能特性提供了简单且经济高效的方法。
