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石胆酸与庆大霉素对革兰阳性菌有协同作用，但对革兰阴性菌没有协同作用。

Synergistic Effect of Lithocholic Acid with Gentamicin against Gram-Positive Bacteria but Not against Gram-Negative Bacteria.

机构信息

Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun 130021, China.

Jilin Ginseng Academy, Chang Chun University of Chinese Medicine, Changchun 130117, China.

出版信息

Molecules. 2022 Apr 3;27(7):2318. doi: 10.3390/molecules27072318.

DOI:10.3390/molecules27072318
PMID:35408717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9000364/
Abstract

() is an important Gram-positive food-borne pathogen that severely threatens public health. A checkerboard microdilution method was performed to evaluate the synergistic effect of lithocholic acid (LCA) with Gentamicin (Genta) against . BacLight LIVE/DEAD staining, scanning electron microscopy and biofilm inhibition assays were further used to explore the bactericidal effect and antibiofilm effect of this combination on . Additionally, the synergistic effects of LCA derivatives with Genta were also evaluated against , and The results indicated that a synergistic bactericidal effect was observed for the combined therapy of LCA at the concentration without affecting bacteria viability, with Genta. Additionally, LCA in combination with Genta had a synergistic effect against Gram-positive bacteria (, and ) but not against Gram-negative bacteria (, and ). BacLight LIVE/DEAD staining and scanning electron microscopy analysis revealed that the combination of LCA with Genta caused membrane injury, leading to bacteria death. We found that 8 μg/mL LCA treatment effectively improved the ability of Genta to eradicate biofilms. In addition, we found that chenodeoxycholic acid, as a cholic acid derivative, also improved the bactericidal effect of Genta against Gram-positive bacteria. Our results indicate that LCA represents a broad-spectrum adjuvant with Genta for infection caused by and other Gram-positive pathogens.

摘要

（）是一种重要的革兰氏阳性食源性病原体，严重威胁公众健康。采用棋盘微量稀释法评价石胆酸（LCA）与庆大霉素（Genta）联合对 的协同作用。进一步采用 BacLight LIVE/DEAD 染色、扫描电子显微镜和生物膜抑制试验探讨该联合用药对 的杀菌作用和抗生物膜作用。此外，还评价了 LCA 衍生物与 Genta 联合对 、 和 的协同作用。结果表明，LCA 在不影响细菌活力的浓度下与 Genta 联合具有协同杀菌作用。此外，LCA 与 Genta 联合对革兰氏阳性菌（ 、 和 ）具有协同作用，但对革兰氏阴性菌（ 、 和 ）没有协同作用。BacLight LIVE/DEAD 染色和扫描电子显微镜分析显示，LCA 与 Genta 联合导致 细胞膜损伤，导致细菌死亡。我们发现 8μg/mL LCA 处理可有效提高 Genta 清除 生物膜的能力。此外，我们发现鹅去氧胆酸作为胆酸衍生物也可提高 Genta 对革兰氏阳性菌的杀菌作用。我们的研究结果表明，LCA 是一种广谱与 Genta 联合应用的佐剂，可用于治疗 及其他革兰氏阳性病原体引起的感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/099bb2face85/molecules-27-02318-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/34f33cd08ded/molecules-27-02318-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/5e33dad2a25b/molecules-27-02318-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/0b4d74b4098c/molecules-27-02318-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/cce247b53416/molecules-27-02318-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/61f79dfaeb9f/molecules-27-02318-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/f1336f468dc9/molecules-27-02318-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/235089b7dc7b/molecules-27-02318-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/e361e46b86cb/molecules-27-02318-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/4740e559b0e5/molecules-27-02318-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/d19cecf065df/molecules-27-02318-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/099bb2face85/molecules-27-02318-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/34f33cd08ded/molecules-27-02318-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/5e33dad2a25b/molecules-27-02318-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/0b4d74b4098c/molecules-27-02318-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/cce247b53416/molecules-27-02318-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/61f79dfaeb9f/molecules-27-02318-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/f1336f468dc9/molecules-27-02318-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/235089b7dc7b/molecules-27-02318-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/e361e46b86cb/molecules-27-02318-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/4740e559b0e5/molecules-27-02318-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/d19cecf065df/molecules-27-02318-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fa7/9000364/099bb2face85/molecules-27-02318-g011.jpg

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