The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China.
Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
ACS Appl Mater Interfaces. 2022 May 4;14(17):19081-19090. doi: 10.1021/acsami.1c22346. Epub 2022 Apr 20.
Single-atom nanozyme (SAzyme) systems have shown great potential in tumor therapy. A multifunctional SAzyme not only possesses high catalytic activity but also can be used as photothermal agents in photothermal therapy (PTT). Furthermore, it is also imperative to overcome tumor thermal resistance in SAzyme-based PTT so that PTT under a mild temperature is achievable. Herein, a novel platelet membrane (PM)-coated mesoporous Fe single-atom nanozyme (Fe-SAzyme) was formulated to solve these issues. The PM-coated mesoporous Fe-SAzyme (PMS) showed a satisfactory NIR-II photothermal performance, high peroxidase (POD) activity, and good tumor-targeting ability. In addition, PMS may be used as a carrier for protein drugs owing to its inner mesoporous structure. experiments showed that PMS could inhibit the expression of heat shock protein (HSP) by damaging the mitochondria, thereby finally improving the effect of mild-temperature PTT. Moreover, results showed that PMS could efficiently accumulate in tumor sites and suppress tumor growth with minimal toxicity in major organs. To the best of our knowledge, this study is the first report of a biomimetic mesoporous Fe-SAzyme used to achieve mitochondrial damage-mediated mild-temperature PTT. The study provides new promising ideas for designing other SAzyme systems for cancer treatment.
单原子纳米酶 (SAzyme) 系统在肿瘤治疗中显示出巨大的潜力。多功能 SAzyme 不仅具有高催化活性，还可用作光热治疗 (PTT) 中的光热剂。此外，克服基于 SAzyme 的 PTT 中的肿瘤热抵抗性也是至关重要的，以便在温和温度下实现 PTT。在此，设计了一种新型血小板膜 (PM) 包覆的介孔 Fe 单原子纳米酶 (Fe-SAzyme) 来解决这些问题。PM 包覆的介孔 Fe-SAzyme (PMS) 表现出令人满意的近红外二区光热性能、高过氧化物酶 (POD) 活性和良好的肿瘤靶向能力。此外，由于其内部介孔结构，PMS 可用作蛋白质药物的载体。实验表明，PMS 可以通过破坏线粒体来抑制热休克蛋白 (HSP) 的表达，从而最终提高温和温度 PTT 的效果。此外，结果表明，PMS 可以有效地在肿瘤部位积聚，并在主要器官中具有最小毒性的情况下抑制肿瘤生长。据我们所知，这项研究是首次报道仿生介孔 Fe-SAzyme 用于实现线粒体损伤介导的温和温度 PTT。该研究为设计用于癌症治疗的其他 SAzyme 系统提供了新的有前途的思路。
