Department of Horticultural Sciences, Texas A&M University, College Station, Texas 77843, USA.
Texas A&M AgriLife Research and Extension Center, Uvalde, Texas 78801, USA.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005311.
A Gram-stain-negative, aerobic and non-spore-forming bacterial strain, designated 20TX0172, was isolated from a rotting onion bulb in Texas, USA. The results of phylogenetic analysis based on the 16S rRNA sequence indicated that the novel strain represented a member of the genus and had the greatest sequence similarities with 520-20 (99.3 %), CFBP 2431 (99.2 %), and 11K1 (99.2 %) but the 16S rRNA phylogenetic tree displayed a monophyletic clade with CFBP 5447. In the phylogenetic trees based on sequences of four housekeeping genes (, , and ), the novel strain formed a separate branch, indicating that the strain was distinct phylogenetically from known species of the genus . The genome-sequence-derived average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the novel isolate and DSM 16733 were 86.7 and 32.7 %, respectively. These values were below the accepted species cutoff threshold of 96 % ANI and 70 % dDDH, affirming that the strain represented a novel species. The genome size of the novel species was 5.98 Mbp with a DNA G+C content of 60.8 mol%. On the basis of phenotypic and genotypic characteristics, strain 20TX0172 represents a novel species of the genus . The name sp. nov. is proposed. The type strain is 20TX0172 (=NCIMB 15426=CIP 112022).
一株革兰氏阴性、需氧、不产芽孢的细菌菌株，被命名为 20TX0172，从美国德克萨斯州腐烂的洋葱鳞茎中分离得到。基于 16S rRNA 序列的系统发育分析结果表明，该新菌株代表 属的一个成员，与 520-20（99.3%）、CFBP 2431（99.2%）和 11K1（99.2%）的相似性最高，但 16S rRNA 系统发育树显示与 CFBP 5447 形成一个单系分支。在基于四个管家基因（、、和 ）序列的系统发育树中，新菌株形成一个单独的分支，表明该菌株在系统发育上与已知的 属物种明显不同。新分离株与 DSM 16733 之间的基于基因组序列的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 86.7%和 32.7%。这些值低于公认的种间界限阈值 96%ANI 和 70%dDDH，证实该菌株代表了一个新的种。新种的基因组大小为 5.98 Mbp，GC 含量为 60.8 mol%。基于表型和基因型特征，菌株 20TX0172 代表了 属的一个新种。建议采用 sp. nov. 这个名称。其模式菌株为 20TX0172（=NCIMB 15426=CIP 112022）。
