Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, People's Republic of China.
Quality Control Department, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, People's Republic of China.
PLoS One. 2022 May 5;17(5):e0267798. doi: 10.1371/journal.pone.0267798. eCollection 2022.
Several studies have demonstrated the association of lncRNAs with a variety of cancers. Here, we explored the role of LINC00922 in gastric cancer (GC) using bioinformatics approaches and in vitro experiments. We examined the expression of LINC00922 and the prognosis of GC patients based on data from The Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA). LINC00922-related genes were identified by the Multi Experiment Matrix (MEM) database and The Atlas of Noncoding RNAs in Cancer (TANRIC), followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction analysis. The significance of LINC00922 in cell proliferation, apoptosis, invasion and migration was assessed by MTT assay, flow cytometry, Transwell assay and wound-healing assay. The expression of LINC00922 was increased in GC tissues compared with adjacent non-tumor tissues, and increased LINC00922 expression was correlated with poor overall survival and disease-free survival. In addition, 336 overlapping genes were identified by the MEM database and TANRIC and found to be involved in GC-related biological processes, such as cell adhesion and migration, as well as TGF-β signaling. In the protein-protein interaction network, hub genes, such as FSTL3 and LAMC1, were identified. LINC00922 overexpression significantly promoted cell proliferation and invasion in vitro, whereas LINC00922 knockdown exerted opposite effects. In summary, our findings indicate that LINC00922 is overexpressed in GC tissues, suggesting that it might play a role in the development and progression of GC, and thus, it might serve as a prognostic indicator of GC.
多项研究表明，lncRNAs 与多种癌症有关。在这里，我们使用生物信息学方法和体外实验探讨了 LINC00922 在胃癌（GC）中的作用。我们根据癌症基因组图谱（TCGA）和基因表达谱交互式分析（GEPIA）的数据，检查了 LINC00922 的表达和 GC 患者的预后。通过多实验矩阵（MEM）数据库和癌症非编码 RNA 图谱（TANRIC），确定了 LINC00922 相关基因，随后进行了基因本体论（GO）、京都基因与基因组百科全书（KEGG）和蛋白质-蛋白质相互作用分析。通过 MTT 测定、流式细胞术、Transwell 测定和划痕愈合测定评估 LINC00922 对细胞增殖、凋亡、侵袭和迁移的意义。与相邻非肿瘤组织相比，GC 组织中 LINC00922 的表达增加，并且增加的 LINC00922 表达与总体生存率和无病生存率差相关。此外，MEM 数据库和 TANRIC 鉴定了 336 个重叠基因，这些基因参与 GC 相关的生物学过程，如细胞粘附和迁移以及 TGF-β 信号转导。在蛋白质-蛋白质相互作用网络中，鉴定了 FSTL3 和 LAMC1 等核心基因。LINC00922 过表达显著促进了体外细胞增殖和侵袭，而 LINC00922 敲低则产生了相反的效果。总之，我们的研究结果表明，LINC00922 在 GC 组织中过度表达，表明它可能在 GC 的发生和发展中发挥作用，因此，它可能成为 GC 的预后指标。
