检测野生和圈养猫科动物中的流产布鲁氏菌。
Molecular detection of Brucella abortus in wild and captive felids.
机构信息
Programa de Pós-Graduação em Medicina Veterinária (PPGVET), Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso (UFMG), Cuiabá, MT, Brazil.
Programa de Pós-Graduação em Medicina Veterinária (PPGVET), Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso (UFMG), Cuiabá, MT, Brazil.
出版信息
Braz J Infect Dis. 2022 Mar-Apr;26(2):102351. doi: 10.1016/j.bjid.2022.102351. Epub 2022 Apr 18.
PURPOSE
Brucellosis is a zoonotic disease of great public health importance. In wild animals, Brucella abortus is one of the most diagnosed species, mainly in enzootic environments where domestic animals share the same environment. B. abortus is common in environments shared by cattle, wild, and domestic animals. This study aimed to detect the presence of B. abortus DNA in free-ranging and captivity felids at Mato Grosso State, Brazil.
METHOD
Polymerase chain reaction, based on the genetic element IS711, was performed in blood samples collected from 23 free-ranging and captive felids. The species represented include Leopardus colocolo, Leopardus pardalis, Leopardus wiedii, Panthera onca, Puma concolor, and Puma yagouaroundi.
RESULTS
DNA amplification of B. abortus was observed in only one captive P. concolor (4.34%).
CONCLUSION
The detection of this pathogen in captive animals using molecular tools demonstrates the importance of monitoring, as it raises concerns about the possibility of transmission between humans and wild and domestic animals, especially in regions of vast biodiversity, such as in the State of Mato Grosso, Brazil.
目的
布鲁氏菌病是一种具有重要公共卫生意义的人畜共患病。在野生动物中，流产布鲁氏菌是最常被诊断出的物种之一，主要存在于家畜和野生动物共享环境的地方。布鲁氏菌在牛、野生动物和家畜共享的环境中很常见。本研究旨在检测巴西马托格罗索州自由放养和圈养猫科动物中是否存在流产布鲁氏菌 DNA。
方法
对从 23 只自由放养和圈养的猫科动物采集的血液样本进行基于遗传元件 IS711 的聚合酶链反应。代表的物种包括 Colocolo 豹猫、豹猫、Wiedii 豹猫、美洲虎、美洲狮和云豹。
结果
仅在一只圈养的美洲狮中观察到流产布鲁氏菌的 DNA 扩增（4.34%）。
结论
使用分子工具在圈养动物中检测到这种病原体，这表明监测的重要性，因为它引起了人们对人类与野生动物和家畜之间传播可能性的关注，特别是在巴西马托格罗索州等生物多样性丰富的地区。
Zotero 插件