脓毒症患者的血液转录组分析揭示了补体C5a受体1基因中的一种长链非编码RNA。
Blood Transcriptome Analysis of Septic Patients Reveals a Long Non-Coding -RNA in the Complement C5a Receptor 1 Gene.
作者信息
Emblem Åse, Knutsen Erik, Jørgensen Tor Erik, Fure Hilde, Johansen Steinar Daae, Brekke Ole-Lars, Mollnes Tom Eirik, Karlsen Bård Ove
机构信息
Research Laboratory and Department of Laboratory Medicine, Nordland Hospital Trust, 8005 Bodø, Norway.
Department of Medical Biology, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
出版信息
Noncoding RNA. 2022 Mar 29;8(2):24. doi: 10.3390/ncrna8020024.
Many severe inflammation conditions are complement-dependent with the complement component C5a-C5aR1 axis as an important driver. At the RNA level, the blood transcriptome undergoes programmed expression of coding and long non-coding RNAs to combat invading microorganisms. Understanding the expression of long non-coding RNAs containing elements in inflammation is important for reconstructing cell fate trajectories leading to severe disease. We have assembled a pipeline for computation mining of new -containing long non-coding RNAs by intersecting immune genes with known coordinates in the human genome. By applying the pipeline to patient bulk RNA-seq data with sepsis, we found immune genes containing 48 insertion as robust candidates for further study. Interestingly, 1 of the 48 candidates was located within the complement system receptor gene C5aR1 and holds promise as a target for RNA therapeutics.
许多严重炎症状态是补体依赖性的，补体成分C5a - C5aR1轴是一个重要驱动因素。在RNA水平上，血液转录组经历编码RNA和长链非编码RNA的程序性表达以对抗入侵的微生物。了解炎症中含特定元件的长链非编码RNA的表达对于重建导致严重疾病的细胞命运轨迹很重要。我们通过将免疫基因与人类基因组中已知的特定坐标相交，组装了一个用于计算挖掘新的含特定元件长链非编码RNA的流程。通过将该流程应用于脓毒症患者的大量RNA测序数据，我们发现含有48个特定插入的免疫基因是进一步研究的有力候选对象。有趣的是，48个候选对象中的1个位于补体系统受体基因C5aR1内，有望成为RNA治疗的靶点。
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