Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
Mar Drugs. 2022 Mar 25;20(4):225. doi: 10.3390/md20040225.
Fucoxanthin, a xanthophyll carotenoid abundant in brown algae, is reported to have several biological functions, such as antioxidant, anti-inflammatory, and anti-tumor activities, in mice. We investigated the effects and mechanisms of fucoxanthin in the mixture oleate/palmitate = 2/1(FFA)-induced nonalcoholic fatty liver disease (NAFLD) cell model in this study. The results showed that the content of superoxide dismutase in the FFA group was 9.8 ± 1.0 U/mgprot, while that in the fucoxanthin high-dose (H-Fx) group (2 μg/mL) increased to 22.9 ± 0.6 U/mgprot. The content of interleukin-1β in the FFA group was 89.3 ± 3.6 ng/mL, while that in the H-Fx group was reduced to 53.8 ± 2.8 ng/mL. The above results indicate that fucoxanthin could alleviate the FFA-induced oxidative stress and inflammatory levels in the liver cells. Oil red-O staining revealed visible protrusions and a significant decrease in the number of lipid droplets in the cytoplasm of cells in the fucoxanthin group. These findings on the mechanisms of action suggest that fucoxanthin can repair FFA-induced NAFLD via the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway and nuclear factor erythroid-2-related factor 2-mediated (Nrf2) signaling pathway, as well as by downregulating the expression of the Toll-like receptor 4-mediated (TLR4) signaling pathway. Fucoxanthin exhibited alleviating effects in the FFA-induced NAFLD model and could be explored as a potential anti-NAFLD substance.
岩藻黄质是一种在褐藻中含量丰富的叶黄素类胡萝卜素，据报道，它在小鼠体内具有多种生物学功能，如抗氧化、抗炎和抗肿瘤活性。本研究旨在探讨岩藻黄质在油酸/棕榈酸（FFA）混合物诱导的非酒精性脂肪性肝病（NAFLD）细胞模型中的作用及其机制。结果表明，FFA 组中超氧化物歧化酶的含量为 9.8±1.0 U/mgprot，而岩藻黄质高剂量（H-Fx）组（2μg/mL）增加到 22.9±0.6 U/mgprot。FFA 组白细胞介素-1β的含量为 89.3±3.6ng/mL，而 H-Fx 组降低至 53.8±2.8ng/mL。上述结果表明，岩藻黄质可以减轻 FFA 诱导的肝细胞氧化应激和炎症水平。油红-O 染色显示，岩藻黄质组细胞的细胞质中可见明显的突起和脂滴数量显著减少。这些作用机制的研究结果表明，岩藻黄质可以通过腺苷单磷酸激活蛋白激酶（AMPK）信号通路和核因子红细胞 2 相关因子 2 介导的（Nrf2）信号通路修复 FFA 诱导的 NAFLD，同时下调 Toll 样受体 4 介导的（TLR4）信号通路的表达。岩藻黄质在 FFA 诱导的 NAFLD 模型中表现出缓解作用，可作为一种潜在的抗 NAFLD 物质进行探索。
