Keawpeng Ittiporn, Paulraj Balaji, Venkatachalam Karthikeyan
Faculty of Agricultural Technology, Songkhla Rajabhat University, Muang, Songkhla 90000, Thailand.
PG and Research Centre in Biotechnology, MGR College, Hosur 635130, Tamil Nadu, India.
Membranes (Basel). 2022 Mar 30;12(4):379. doi: 10.3390/membranes12040379.
Mung bean () flour serves as an excellent biopolymer and a potential material for producing antioxidant and antimicrobial phyto-films. In addition to mung bean flour, this study also combined the longkong (Aglaia dookkoo Griff.) pericarp extract (LPE, 1.5%) and ultrasonication process (0 (C1), 2 (T1), 4 (T2), 6 (T3), 8 (T4), and 10 (T5) min, sonicated at 25 kHz, 100% amplitude) in film emulsion production to improve the antioxidant and antimicrobial efficiency in the phyto-films. This study showed that sonication increased the phyto-films' color into more lightness and yellowness, and the intensity of the color changes was in accordance with the increased sonication time. Alternatively, the thickness, water vapor permeability, and solubility of the films were adversely affected by extended sonication. In addition, elongation at break and tensile strength increased while the Young modulus decreased in the phyto-films with the extended sonication. Furthermore, the droplet size and polydispersity index of the phyto-films decreased with extended sonication. Conversely, the zeta potential of the film tended to increase with the treatments. Furthermore, phytochemicals such as total phenolic content and total flavonoid contents, and the radical scavenging ability of phyto-films against the DPPH radical, ABTS radical, superoxide radical, hydroxyl radical, and ferrous chelating activity, were significantly higher, and they were steadily increased in the films with the extended sonication time. Furthermore, the phyto-films showed a significant control against Gram (-) pathogens, followed by Gram (+) pathogens. A higher inhibitory effect was noted against L. monocytogens, followed by and . Similarly, the phyto-films also significantly inhibited the Gram (-) pathogens, and significant control was noted against , followed by and . Regardless of the mung bean flour, this study found that longkong pericarp extract and the sonication process could also effectively be used in the film emulsions to enhance the efficiency of the antioxidant and antimicrobial properties of phyto-films.
绿豆粉是一种优质的生物聚合物，也是生产具有抗氧化和抗菌性能的植物薄膜的潜在材料。除了绿豆粉，本研究还在薄膜乳液生产中加入了龙贡（Aglaia dookkoo Griff.）果皮提取物（LPE，1.5%）和超声处理过程（0（C1）、2（T1）、4（T2）、6（T3）、8（T4）和10（T5）分钟，在25 kHz、100%振幅下超声处理），以提高植物薄膜的抗氧化和抗菌效率。本研究表明，超声处理使植物薄膜的颜色更浅且更黄，颜色变化的强度与超声处理时间的增加一致。另外，延长超声处理时间会对薄膜的厚度、水蒸气透过率和溶解度产生不利影响。此外，随着超声处理时间延长，植物薄膜的断裂伸长率和拉伸强度增加，而杨氏模量降低。此外，植物薄膜的液滴尺寸和多分散指数随着超声处理时间延长而减小。相反，薄膜的zeta电位倾向于随着处理而增加。此外，植物薄膜中的总酚含量、总黄酮含量等植物化学物质以及对DPPH自由基、ABTS自由基、超氧自由基、羟基自由基的自由基清除能力和亚铁螯合活性显著更高，并且随着超声处理时间延长在薄膜中稳步增加。此外，植物薄膜对革兰氏阴性病原体有显著抑制作用，其次是革兰氏阳性病原体。对单核细胞增生李斯特菌的抑制作用更高，其次是[此处原文缺失两种菌名]和[此处原文缺失两种菌名]。同样，植物薄膜也显著抑制革兰氏阴性病原体，对[此处原文缺失两种菌名]有显著抑制作用，其次是[此处原文缺失两种菌名]和[此处原文缺失两种菌名]。无论有无绿豆粉，本研究发现龙贡果皮提取物和超声处理过程也可有效地用于薄膜乳液中，以提高植物薄膜的抗氧化和抗菌性能效率。
