Shenouda Mary L, Ambilika Maria, Skellam Elizabeth, Cox Russell J
Institute for Organic Chemistry and Biomolekulares Wirkstoffzentrum (BMWZ), Schneiderberg 38, 30167 Hannover, Germany.
Pharmacognosy Department, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
J Fungi (Basel). 2022 Mar 30;8(4):355. doi: 10.3390/jof8040355.
() was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, ) from and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, ) from . The resulting engineered strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.
（某菌株）被开发为用于真菌次级代谢产物异源表达的微生物细胞工厂。这是通过灭活山梨素类生物合成以及构建用于快速克隆和表达异源真菌生物合成基因的载体来实现的。使用了两种类型的巨型合成酶来测试该菌株和载体，即来自（某菌）的非还原型聚酮合酶（nr-PKS，某编号）和来自（另一菌）的杂合高还原型聚酮合酶-非核糖体肽合成酶（hr-PKS-NRPS，某编号）。所得的工程化（该菌株）能够在包括马铃薯、橙子、香蕉和猕猴桃皮以及大麦秸秆在内的废料上产生预期的天然产物3-甲基原儿茶醛和前体萘菌素A。将（该菌株）开发为用于次级代谢产物生产的异源宿主代表了一种通过将废弃生物质直接转化为次级代谢产物来实现废物增值的新方法。
