Guo Mengmeng, Cao Xi, Zhang Ke, Pan Menghao, Wu Yujiang, Langda Suo, Yang Yuxin, Chen Yulin, Gui Ba, Ma Baohua
Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yanglin 712100, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yanglin 712100, China.
Vet Sci. 2022 Apr 1;9(4):169. doi: 10.3390/vetsci9040169.
The gut microbiota play a vital role in regulating endocrine-mediated metabolism, immunity, and energy metabolism. However, little is known about the gut microbiota and metabolite composition and development throughout pregnancy and lactation. Here, we used amplicon sequencing to analyze the gut microbiota composition of 35 female mice in five stages of pregnancy and lactation, namely, non-receptive (NR) stages, sexually-receptive (SR) stages, at day 15 of pregnancy (Pre_D15), at the day of birth (Del), and at day 10 of lactation (Lac_D10). The results revealed that the α diversity of gut microbiota was significantly increased during pregnancy and lactation. In addition, the principal coordinate analysis (PCoA) conducted on the amplicon sequence variants' (ASVs') distribution of the 16S rRNA amplicons indicated that the microbiota composition was significantly different among the five groups. Based on a random forest analysis, , and were the most important microbiota. The abundance of , and were negatively correlated with the serum progesterone concentration, while the abundance of was positively correlated with the propionate and valerate concentration in the caecal contents. Moreover, the concentration of acetate and propionate in the Del and Lac_D10 stages was significantly lower than in the SR and Pre_D15 stages. Our findings indicate that some gut microbes and metabolites vary considerably at the different stages of pregnancy and during lactation stages, which can potentially be used as microbial biomarkers. These results provide information on the potential use of the identified microbes as probiotics to maintain a healthy pregnancy and lactation.
肠道微生物群在调节内分泌介导的代谢、免疫和能量代谢方面发挥着至关重要的作用。然而，关于整个妊娠和哺乳期的肠道微生物群及其代谢物组成和发育情况，我们所知甚少。在此，我们使用扩增子测序分析了35只雌性小鼠在妊娠和哺乳期五个阶段的肠道微生物群组成，这五个阶段分别为非接受期（NR）、性接受期（SR）、妊娠第15天（Pre_D15）、出生日（Del）和哺乳第10天（Lac_D10）。结果显示，妊娠和哺乳期肠道微生物群的α多样性显著增加。此外，对16S rRNA扩增子的扩增子序列变体（ASV）分布进行的主坐标分析（PCoA）表明，五组之间的微生物群组成存在显著差异。基于随机森林分析， 、 和 是最重要的微生物群。 、 和 的丰度与血清孕酮浓度呈负相关，而 的丰度与盲肠内容物中丙酸和戊酸的浓度呈正相关。此外，Del和Lac_D10阶段的乙酸盐和丙酸盐浓度显著低于SR和Pre_D15阶段。我们的研究结果表明，一些肠道微生物和代谢物在妊娠和哺乳的不同阶段有很大差异，这有可能被用作微生物生物标志物。这些结果为将所鉴定的微生物用作益生菌以维持健康的妊娠和哺乳期提供了潜在用途的信息。
