Wilson Jens, Swanbeck Sarah, Banning Gavin, Alhwayek Tatiana, Sullivan Victoria, Howard Katherine M, Kingsley Karl
Department of Advanced Education in Pediatric Dentistry, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W, Charleston Boulevard, Las Vegas, NV 89106, USA.
Department of Clinical Sciences, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W, Charleston Boulevard, Las Vegas, NV 89106, USA.
Methods Protoc. 2022 Apr 7;5(2):31. doi: 10.3390/mps5020031.
Silver diamine fluoride (SDF) has been useful in clinical dentistry for the purpose of caries arrest and prevention. Although methods for the application of SDF are well-known among dental professionals, such as microbrush applications, few studies have explored the effect of light curing, which accelerates precipitation onto dentin, and whether this has any effect on the antimicrobial properties of SDF. To assess this technique, single () and polymicrobial (mixed salivary) colonies were grown and plated using SDF applied to hydroxyapatite discs with and without treatment with curing light. Kirby-Bauer Zone of Inhibition assay results revealed no significant differences in the areas between the two treatment groups (SDF: 1.27 mm, SDF plus curing light: 1.25 mm), = 0.887 in the single culture () experiments. In addition, no significant differences were found between the two treatment groups (SDF: 1.26 mm, SDF plus curing light: 1.24 mm), = 0.771 in the polymicrobial culture experiments. Although there may be specific properties associated with SDF induced following light curing, these differences do not appear to be associated with the antimicrobial properties affecting gram-positive or polymicrobial films.
氟化亚锡（SDF）在临床牙科中已被用于龋齿的控制和预防。尽管牙科专业人员对SDF的应用方法（如微刷涂抹）很熟悉，但很少有研究探讨光固化（可加速其在牙本质上的沉淀）的效果，以及这是否会对SDF的抗菌性能产生影响。为了评估这项技术，使用SDF分别处理有和没有经过固化光处理的羟基磷灰石圆盘，培养并接种单一（）和多微生物（混合唾液）菌落。Kirby-Bauer抑菌圈试验结果显示，在单一培养（）实验中，两个处理组之间的抑菌圈面积没有显著差异（SDF组：1.27毫米，SDF加固化光组：1.25毫米），P = 0.887。此外，在多微生物培养实验中，两个处理组之间也没有发现显著差异（SDF组：1.26毫米，SDF加固化光组：1.24毫米），P = 0.771。尽管光固化后诱导产生的SDF可能具有特定性质，但这些差异似乎与影响革兰氏阳性菌或多微生物生物膜的抗菌性能无关。
