Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Human Physiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Sci Rep. 2022 Apr 21;12(1):6555. doi: 10.1038/s41598-022-10402-w.
Even with considerable progress in cancer researches, gastric cancer is still one of the global health problems. Recognition of the differential expressed genes in GC is the most appropriate approach for establishing new diagnostic targets. This study evaluates SEC13, SMAD7, GHRL, lncRNA GHRLOS, HIF-1α genes profiling as well as HIF-1α protein level for GC. The expression of selected genes, serum HIF-1α and CEA protein levels were determined for 50 GC patients and 50 healthy controls by real-time RT-PCR, ELISA, and ELICA respectively. The sensitivities of these parameters as diagnostic biomarkers were evaluated. SMAD7, HIF-1α expression, serum HIF-1α, and CEA level were significantly upregulated in GC patients as compared to the control group (P = 0.024, < 0.001) and had significant positive correlations between each other except SMAD7 with serum HIF-1α, and CEA level. On the other hand, SEC13, GHRL, and lncRNA GHRLOS expression were significantly downregulated in GC patients (P = < 0.001, 0.025, < 0.001 respectively) and had significant positive correlations with each other (P < 0.001). Significant negative correlations were observed between most of both groups. All studied parameters were associated with GC clinical stages except SMAD7 was associated with stage IV only (P = 0.005) and GHRL did not associate with tumor stages (P ˃ 0.05). All studied parameters may be promising biomarkers for the early diagnosis of GC. SMAD7, HIF-1α gene, and HIF-1α protein may be jointly implicated in cancer development and prognosis, while SEC13, GHRL, and lncRNA GHRLOS may act as tumor suppressors.
即使在癌症研究方面取得了相当大的进展，胃癌仍然是全球健康问题之一。识别 GC 中的差异表达基因是建立新的诊断靶点的最合适方法。本研究评估了 SEC13、SMAD7、GHRL、lncRNA GHRLOS 和 HIF-1α 基因谱以及 GC 的 HIF-1α 蛋白水平。通过实时 RT-PCR、ELISA 和 ELICA 分别测定了 50 例 GC 患者和 50 例健康对照者选定基因的表达、血清 HIF-1α 和 CEA 蛋白水平。评估了这些参数作为诊断生物标志物的敏感性。与对照组相比，GC 患者的 SMAD7、HIF-1α 表达、血清 HIF-1α 和 CEA 水平显着上调（P = 0.024，< 0.001），且彼此之间呈显着正相关，除 SMAD7 与血清 HIF-1α 和 CEA 水平之间无相关性外。另一方面，GC 患者 SEC13、GHRL 和 lncRNA GHRLOS 表达显着下调（P = < 0.001，0.025，< 0.001），且彼此之间呈显着正相关（P < 0.001）。两组之间大多数都观察到显着的负相关。除 SMAD7 仅与 IV 期相关（P = 0.005）和 GHRL 与肿瘤分期无关（P ˃ 0.05）外，所有研究参数均与 GC 临床分期相关。所有研究参数都可能是 GC 的早期诊断有希望的生物标志物。SMAD7、HIF-1α 基因和 HIF-1α 蛋白可能共同参与癌症的发生和预后，而 SEC13、GHRL 和 lncRNA GHRLOS 可能作为肿瘤抑制因子。
