Department of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050019, P.R. China.
Examination and Training Center, Health and Family Planning Commission of Hebei, Shijiazhuang, Hebei 050051, P.R. China.
Mol Med Rep. 2019 Sep;20(3):2875-2881. doi: 10.3892/mmr.2019.10531. Epub 2019 Jul 25.
Long intergenic non‑protein coding RNA, p53 induced transcript (Linc‑pint) has been reported to be downregulated in various cancer cell lines; however, its expression profile and role in gastric cancer remains unknown. The present study aimed to investigate the involvement of Linc‑pint in gastric cancer. Through quantitative polymerase chain reaction, western blotting and viability assays, it was observed that Linc‑pint expression was significantly downregulated in gastric biopsies from patients with gastric cancer, compared with healthy controls. Conversely, the expression of hypoxia‑inducible factor‑1α (HIF‑1α) mRNA was significantly upregulated in patients with gastric cancer compared with in healthy controls. Using a variety of statistical inference tests, including receiver operating characteristic curve and correlation analyses, it was determined that the expression levels of Linc‑pint and HIF‑1α exhibited a significantly negative correlation in patients with gastric cancer but not in healthy controls. Linc‑pint expression was significantly and inversely associated with tumor size but not tumor metastasis. Linc‑pint overexpression inhibited the proliferation of gastric cancer cells, whereas treatment with exogenous HIF‑1α promoted proliferation. Linc‑pint overexpression downregulated the expression of HIF‑1α, whereas exogenous HIF‑1α did not significantly alter Linc‑pint expression. Furthermore, treatment with exogenous HIF‑1α suppressed the inhibitory effects of Linc‑pint overexpression on the proliferation of gastric cancer cells. In conclusion, overexpression of Linc‑pint may inhibit the growth of gastric tumors via downregulation of HIF‑1α.
长链非编码 RNA，p53 诱导转录物（Linc-pint）已被报道在各种癌细胞系中下调；然而，其在胃癌中的表达谱和作用尚不清楚。本研究旨在探讨 Linc-pint 在胃癌中的作用。通过定量聚合酶链反应、蛋白质印迹和活力测定，观察到 Linc-pint 在胃癌患者的胃活检组织中表达明显下调，与健康对照组相比。相反，与健康对照组相比，胃癌患者缺氧诱导因子-1α（HIF-1α）mRNA 的表达明显上调。通过多种统计推断检验，包括接收者操作特征曲线和相关性分析，确定 Linc-pint 和 HIF-1α 的表达水平在胃癌患者中呈显著负相关，但在健康对照组中无显著相关性。Linc-pint 的表达与肿瘤大小显著负相关，但与肿瘤转移无关。Linc-pint 的过表达抑制了胃癌细胞的增殖，而外源性 HIF-1α促进了增殖。Linc-pint 的过表达下调了 HIF-1α 的表达，而外源性 HIF-1α对 Linc-pint 的表达没有显著影响。此外，外源性 HIF-1α 处理抑制了 Linc-pint 过表达对胃癌细胞增殖的抑制作用。综上所述，Linc-pint 的过表达可能通过下调 HIF-1α 抑制胃肿瘤的生长。
