能量和宏量营养素摄入与中年久坐成年人 S-Klotho 血浆水平的关系：一项横断面研究。

Association of Energy and Macronutrients Intake with S-Klotho Plasma Levels in Middle-Aged Sedentary Adults: A Cross-Sectional Study.

机构信息

Lucas Jurado-Fasoli, EFFECTS 262 Research Group, Department of Medical Physiology, School of Medicine. University of Granada; 18071 Granada, Spain; E.mail:

出版信息

J Nutr Health Aging. 2022;26(4):360-366. doi: 10.1007/s12603-022-1763-2.

DOI:10.1007/s12603-022-1763-2

PMID:35450992

Abstract

BACKGROUND

The ageing process can be influenced by energy intake and different macronutrients within the diet. The soluble form of the α-Klotho gene (called S-Klotho) is widely considered as a powerful anti-ageing biomarker.

OBJECTIVE

To analyze the association of energy, dietary energy density and macronutrient intake with S-Klotho plasma levels in middle-aged sedentary adults.

METHODS

A total of 72 (52.8% women) middle-aged sedentary adults (53.7 ± 5.2 years old) participated in the study. Energy and macronutrients intake (i.e. fat, carbohydrate and protein) were assessed using three non-consecutive 24-h recalls. S-Klotho plasma levels were measured in the Ethylenediaminetetraacetic acid (EDTA) plasma using a solid-phase sandwich enzyme-linked immunosorbent assay.

RESULTS

No association was observed between energy, dietary energy density or macronutrient intake and S-Klotho plasma levels in men (all P≥ 0.1). We found an inverse association between energy, protein and carbohydrate intake with S-Klotho plasma levels in women (all P≤0.043), which disappeared after controlling for age, lean mass index and sedentary time. An inverse association was observed between dietary energy density and S-Klotho plasma levels in women after controlling for covariates (all P≤0.05).

CONCLUSION

In summary, the present study showed an inverse association of dietary energy density with S-Klotho plasma levels in middle-aged women. In addition, our data suggest that the associations between energy and macronutrient intake could be highly dependent on lean mass and sedentary time.

摘要

背景

能量摄入和饮食中的不同宏量营养素会影响衰老过程。α-klotho 基因的可溶性形式（称为 S-Klotho）被广泛认为是一种强大的抗衰老生物标志物。

目的

分析中年久坐成年人的能量、饮食能量密度和宏量营养素摄入与 S-Klotho 血浆水平之间的关系。

方法

共有 72 名（52.8%为女性）中年久坐成年人（53.7±5.2 岁）参与了这项研究。使用三份非连续的 24 小时回顾法评估能量和宏量营养素（即脂肪、碳水化合物和蛋白质）的摄入量。使用固相夹心酶联免疫吸附试验在乙二胺四乙酸（EDTA）血浆中测量 S-Klotho 血浆水平。

结果

在男性中，未观察到能量、饮食能量密度或宏量营养素摄入与 S-Klotho 血浆水平之间存在关联（所有 P≥0.1）。我们发现女性的能量、蛋白质和碳水化合物摄入与 S-Klotho 血浆水平之间存在负相关（均 P≤0.043），但在控制年龄、瘦体重指数和久坐时间后，这种相关性消失了。在控制了协变量后，女性的饮食能量密度与 S-Klotho 血浆水平之间也存在负相关（均 P≤0.05）。