四种致病真菌对两性霉素B和两性霉素B甲酯的敏感性比较
Comparative susceptibility of four kinds of pathogenic fungi to amphotericin B and amphotericin B methyl ester.
作者信息
Huston A C, Hoeprich P D
出版信息
Antimicrob Agents Chemother. 1978 Jun;13(6):905-9. doi: 10.1128/AAC.13.6.905.
The activity of amphotericin B methyl ester was compared with that of amphotericin B, using Candida albicans (34 isolates), Torulopsis glabrata (12 isolates), Filobasidiella neoformans (stat. conid. Cryptococcus neoformans) (14 isolates), and Coccidioides immitis (37 isolates) and tube dilution in a totally synthetic, completely defined medium (SAAMF) with inocula of 10(4) colony-forming units per ml. Minimal inhibitory concentrations were read after 24 h at 34 degrees C for C. albicans and T. glabrata, and after 48 h at 34 degrees C for C. immitis and F. neoformans. Minimal lethal concentrations were determined by subculture of 10% of the volume of the cultures without evident growth onto Sabouraud glucose agar medium. Overall, amphotericin B methyl ester was slightly less active than amphotericin B, with the differences attaining statistical significance for: (i) inhibition of C. albicans and T. glabrata and (ii) killing of T. glabrata.
使用白色念珠菌（34株分离株）、光滑球拟酵母（12株分离株）、新型隐球菌（原称新生隐球菌，14株分离株）和粗球孢子菌（37株分离株），在一种完全合成的、成分明确的培养基（SAAMF）中，接种量为每毫升10⁴个菌落形成单位，通过试管稀释法比较两性霉素B甲酯与两性霉素B的活性。对于白色念珠菌和光滑球拟酵母，在34℃培养24小时后读取最低抑菌浓度；对于粗球孢子菌和新型隐球菌，在34℃培养48小时后读取最低抑菌浓度。通过将无明显生长的培养物体积的10%转接至沙氏葡萄糖琼脂培养基来确定最低致死浓度。总体而言，两性霉素B甲酯的活性略低于两性霉素B，以下方面的差异具有统计学意义：（i）对白色念珠菌和光滑球拟酵母的抑制作用；（ii）对光滑球拟酵母的杀灭作用。
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