Hoeprich P D, Huston A C
J Infect Dis. 1976 Oct;134(4):336-41. doi: 10.1093/infdis/134.4.336.
The capacity of four culture media to obfuscate the antifungal activity of miconazole and amphotericin B methyl ester was evaluated qualitatively by examination of five isolates each of Candida albicans, Candida tropicalis, Candida parapsilosis, Torulopsis glabrata, and Cryptococcus neoformans, and quantitatively by determination of the absolute minimal inhibitory concentrations for a strain of C. albicans. Miconazole, like the predecessor imidazole (clotrimazole), was antagonized by two complex, undefined media (Sabouraud's glucose and brain-heart infusion agars) but not by either of two synthetic formulations (synthetic amino acid medium, fungal and modified yeast-nitrogen base). The antifungal activity of amphotericin B methyl ester, like that of the parent compound amphotericin B, was not materially affected by the culture medium used for testing. When added separately to synthetic amino acid medium (fungal), neither purines nor pyrimidines antagonized miconazole. Ether extraction of Sabouraud's glucose agar failed to diminish the antagonism of this medium for miconazole.
通过检测白色念珠菌、热带念珠菌、近平滑念珠菌、光滑球拟酵母菌和新型隐球菌各5个分离株，定性评估了4种培养基对咪康唑和两性霉素B甲酯抗真菌活性的影响，并通过测定白色念珠菌菌株的绝对最低抑菌浓度进行定量评估。咪康唑与前体咪唑（克霉唑）一样，受到两种复杂的、成分未明的培养基（沙氏葡萄糖琼脂和脑心浸液琼脂）的拮抗，但未受到两种合成培养基（合成氨基酸培养基、真菌和改良酵母氮碱）中任何一种的拮抗。两性霉素B甲酯的抗真菌活性，与母体化合物两性霉素B一样，所使用的测试培养基对其没有实质性影响。当分别添加到合成氨基酸培养基（真菌）中时，嘌呤和嘧啶均未拮抗咪康唑。对沙氏葡萄糖琼脂进行乙醚萃取，未能减弱该培养基对咪康唑的拮抗作用。
