Saubolle M A, Hoeprich P D
Antimicrob Agents Chemother. 1978 Oct;14(4):517-30. doi: 10.1128/AAC.14.4.517.
A disk agar diffusion method was developed for testing the susceptibility of rapidly growing yeasts in vitro. A totally defined, completely synthetic agar culture medium (synthetic amino acid medium, fungal) and clinical isolates of Candida spp. and Torulopsis glabrata were used. Turbidimetric adjustment of cell suspensions resulted in standard, reproducible inocula, which gave sharp, clear zones of inhibition when applied by an agar overlay method. Optimal disk loads were determined for amphotericin B, amphotericin B methyl ester, 5-fluorocytosine, clotrimazole, and miconazole. Disk potencies were stable over a 2-month period when stored in a vacuum desiccator at -30 degrees C. Using an error ratebounded classification, the zones of inhibition were correlated with both broth dilution and agar dilution minimum inhibitory concentrations (MICs). With amphotericin B and amphotericin B methyl ester, all isolates were susceptible, yielding zone diameters which clustered within 5 mm. Overall correlations between zone diameters and broth dilution MICs with 5-fluorocytosine, miconazole, and clotrimazole were 97, 96, and 82% (excluding T. glabrata), respectively; correlations of zone diameters with agar dilution MICs were 96, 92, and 88%, respectively. Disk diffusion susceptibility testing of yeasts appears to be generally applicable. However, when results are equivocal, quantitative test methods should be used.
开发了一种纸片琼脂扩散法用于体外检测快速生长酵母的敏感性。使用了一种完全确定的、完全合成的琼脂培养基（合成氨基酸培养基，真菌用）以及念珠菌属和光滑假丝酵母的临床分离株。通过比浊法调整细胞悬液得到标准的、可重复的接种物，采用琼脂覆盖法接种时可产生清晰、明显的抑菌圈。确定了两性霉素B、两性霉素B甲酯、5-氟胞嘧啶、克霉唑和咪康唑的最佳纸片载药量。纸片效力在-30℃真空干燥器中储存2个月期间保持稳定。使用误差率受限分类法，抑菌圈与肉汤稀释法和琼脂稀释法最低抑菌浓度（MIC）相关。对于两性霉素B和两性霉素B甲酯，所有分离株均敏感，抑菌圈直径聚集在5mm范围内。5-氟胞嘧啶、咪康唑和克霉唑的抑菌圈直径与肉汤稀释法MIC的总体相关性分别为97%、96%和82%（不包括光滑假丝酵母）；抑菌圈直径与琼脂稀释法MIC的相关性分别为96%、92%和88%。酵母的纸片扩散药敏试验似乎普遍适用。然而，当结果不明确时，应使用定量检测方法。
