Kumar Rahul, Porter Robert E, Mor Sunil K, Goyal Sagar M
Veterinary Diagnostic Laboratory, Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Ave, Saint Paul, MN 55108, USA.
Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyaya Veterinary Science University and Cattle Research Institute, Mathura 281001, Uttar Pradesh, India.
Vaccines (Basel). 2022 Mar 22;10(4):486. doi: 10.3390/vaccines10040486.
We created a recombinant live pichinde virus-vectored bivalent codon optimized subunit vaccine that expresses immunogenic Sigma C and Sigma B proteins of turkey arthritis reovirus. The vaccine virus could be transmitted horizontally immunizing the non-vaccinated pen mates. The vaccine was tested for efficacy against homologous (TARV SKM121) and heterologous (TARV O'Neil) virus challenge. Immunized poults produced serum neutralizing antibodies capable of neutralizing both viruses. The vaccinated and control birds showed similar body weights indicating no adverse effect on feed efficiency. Comparison of virus gene copy numbers in intestine and histologic lesion scores in tendons of vaccinated and non-vaccinated birds showed a decrease in the replication of challenge viruses in the intestine and tendons of vaccinated birds. These results indicate the potential usefulness of this vaccine.
我们构建了一种重组活皮钦德病毒载体二价密码子优化亚单位疫苗，该疫苗表达火鸡关节炎呼肠孤病毒具有免疫原性的西格玛C和西格玛B蛋白。疫苗病毒能够水平传播，从而使未接种疫苗的同栏伙伴获得免疫。对该疫苗针对同源病毒（火鸡关节炎呼肠孤病毒SKM121）和异源病毒（火鸡关节炎呼肠孤病毒奥尼尔株）攻击的效力进行了测试。免疫后的雏禽产生了能够中和这两种病毒的血清中和抗体。接种疫苗和未接种疫苗的禽类体重相似，表明对饲料效率没有不良影响。对接种疫苗和未接种疫苗禽类的肠道病毒基因拷贝数以及肌腱组织学病变评分进行比较，结果显示接种疫苗禽类的肠道和肌腱中攻击病毒的复制有所减少。这些结果表明该疫苗具有潜在的应用价值。
