State Key Laboratory of Hybrid Rice, Hubei Hongshan Laboratory, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Int J Mol Sci. 2022 Apr 18;23(8):4444. doi: 10.3390/ijms23084444.
High light intensity as one of the stresses could lead to generation of large amounts of reactive oxygen species (ROS) in plants, resulting in severe plant growth retardation. The photorespiration metabolism plays an important role in producing and removing a variety of ROS, maintaining the dynamic balance of the redox reaction, and preventing photoinhibition. hydroxypyruvate reductase 1 (HPR1) is a primary metabolic enzyme in the photorespiration cycle. However, the role of HPR1 in plants response to high light is not clear. Here, we found that the expression of could be induced by high light intensity. The growth and photosynthetic capacity of mutants are seriously affected under high light intensity. The absence of HPR1 suppresses the rates of photorepair of Photosystem II (PSII), aggravates the production of ROS, and accelerates photorespiration rates. Moreover, the activity of ROS scavenging enzymes in the mutants is significantly higher. These results indicate that HPR1 is involved in plant response to high light intensity and is essential for maintaining the dynamic balance of ROS and photorespiration.
高光强作为一种胁迫因素，可导致植物中大量活性氧（ROS）的产生，从而严重抑制植物生长。光呼吸代谢在产生和清除各种 ROS、维持氧化还原反应的动态平衡以及防止光抑制方面起着重要作用。羟丙酮磷酸还原酶 1（HPR1）是光呼吸循环中的主要代谢酶。然而，HPR1 在植物对高光强的响应中的作用尚不清楚。在这里，我们发现可以被高光强诱导。在高光强下，突变体的生长和光合作用能力受到严重影响。HPR1 的缺失抑制了光系统 II（PSII）的光修复速率，加剧了 ROS 的产生，并加速了光呼吸速率。此外，突变体中 ROS 清除酶的活性显著升高。这些结果表明，HPR1 参与了植物对高光强的响应，对于维持 ROS 和光呼吸的动态平衡是必不可少的。
