Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy.
Division of Laboratory Medicine, University Hospital Giuliano Isontina, 34149 Trieste, Italy.
Viruses. 2022 Mar 29;14(4):704. doi: 10.3390/v14040704.
Coronavirus disease 19 (COVID-19) clinical manifestations include the involvement of the gastrointestinal tract, affecting around 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected children. In the present work, the consequence of a short time of viral absorption (5, 15, 30 and 60 min) was tested on the Caco-2 intestinal epithelial cell line. Our findings show that Caco-2 cells are highly permissive to SARS-CoV-2 infection, even after 5 min of viral inoculation at a multiplicity of infection of 0.1. No cytopathic effect was evident during the subsequent 7 days of monitoring; nevertheless, the immunofluorescence staining for the viral nucleocapsid confirmed the presence of intracellular SARS-CoV-2. Our findings highlight the very short time during which SARS-CoV-2 is able to infect these cells in vitro.
新型冠状病毒病（COVID-19）的临床表现包括胃肠道受累，约影响 10%严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）感染的儿童。在本工作中，我们测试了病毒吸收（5、15、30 和 60 分钟）短时间对 Caco-2 肠上皮细胞系的影响。我们的研究结果表明，Caco-2 细胞对 SARS-CoV-2 感染高度易感，即使在感染复数为 0.1 时病毒接种 5 分钟后也是如此。在随后的 7 天监测过程中，没有明显的细胞病变效应；然而，针对病毒核衣壳的免疫荧光染色证实了细胞内 SARS-CoV-2 的存在。我们的研究结果强调了 SARS-CoV-2 在体外感染这些细胞的极短时间。
