Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
Global Virus Network Center of Excellence at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
Viruses. 2022 Mar 29;14(4):715. doi: 10.3390/v14040715.
The COVID-19 pandemic caused by SARS-CoV-2 is having devastating effects on a global scale. Since common household disinfectants are often used to minimise the risk of infection in the home and work environment, we investigated the ability of some of these products to inactivate the virus. We tested generic brands of vinegar, bleach, and dishwashing detergent, as well as laboratory-grade acetic acid, sodium hypochlorite, and ethanol. Assays were conducted at room temperature (18-20 °C, 40% relative humidity), and two time points were used to reflect a quick wipe (30 s) and a brief soak (5 min). Vinegar, and its active ingredient, acetic acid, were completely ineffective at virus inactivation even when exposed to the virus at 90% (a final concentration equivalent to 3.6% acetic acid). In contrast, ethanol was capable of inactivating the virus at dilutions as low as 40% . Dishwashing detergent effectively rendered SARS-CoV-2 inactive when diluted 100-fold (1% ). Bleach was found to be fully effective against SARS-CoV-2 at 0.21 g/L sodium hypochlorite after a 30 s exposure (1/200 dilution of commercial product). Given reports of infectious virus recovered from the surface of frozen packaging, we tested the persistence of infectiousness after multiple freeze-thaw cycles and found no change in infectious SARS-CoV-2 titre after seven freeze-thaw cycles. These results should help inform readers of how to effectively disinfect surfaces and objects that have potentially been contaminated with SARS-CoV-2 using common household chemicals.
由 SARS-CoV-2 引起的 COVID-19 大流行正在全球范围内造成毁灭性影响。由于常用的家用消毒剂通常用于降低家庭和工作环境中的感染风险，因此我们研究了其中一些产品灭活病毒的能力。我们测试了普通品牌的醋、漂白剂和洗碗剂，以及实验室级别的乙酸、次氯酸钠和乙醇。在室温（18-20°C，40%相对湿度）下进行检测，并使用两个时间点来反映快速擦拭（30 秒）和短暂浸泡（5 分钟）。即使将醋及其有效成分乙酸以 90%的浓度（最终浓度相当于 3.6%的乙酸）暴露于病毒下，醋和乙酸也完全无法使病毒失活。相比之下，乙醇在 40%的低浓度下就能有效灭活病毒。当稀释 100 倍（1%）时，洗碗剂能有效使 SARS-CoV-2 失活。鉴于有报道称在冷冻包装的表面上回收了具有传染性的病毒，我们测试了多次冻融循环后传染性的持久性，在经过 7 次冻融循环后，传染性 SARS-CoV-2 滴度没有变化。这些结果应该有助于读者了解如何使用常见的家用化学品有效消毒可能已被 SARS-CoV-2 污染的表面和物品。
