Ryerson University, Department of Chemistry and Biology; Toronto, Ontario, Canada, M5B 2K3.
Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
Am J Infect Control. 2022 Mar;50(3):325-329. doi: 10.1016/j.ajic.2021.10.021. Epub 2021 Oct 29.
The COVID-19 pandemic has had an unprecedented impact on global health and the world's economies. Proliferation of virulent and deadly SARS-CoV-2 variants require effective transmission mitigation strategies. Under reasonable environmental conditions, culturable and infectious SARS-CoV-2 can survive on contaminated fomites from hours to months. In the present study we evaluated a surface-anchored polymeric quaternary ammonium antimicrobial to help reduce fomite transmission of SARS-CoV-2 from contaminated surfaces.
Two studies were performed on antimicrobial pre-treated metal disks in March 2020 by two independent Biosafety Level III (BSL-3) equipped laboratories in April 2020. These facilities were in Belgium (the Rega Medical Research Institute) and Australia (the Peter Doherty Institute) and independently applied quantitative carrier-based methodologies using the authentic SARS-CoV-2 isolates (hCoV-19/Australia/VIC01/2020, hCoV-19/Belgium/GHB-03021/2020).
Residual dry tests were independently conducted at both facilities and demonstrated sustained virion destruction (108.23 TCID50/carrier GHB-03021 isolate, and 103.66 TCID50/carrier VIC01 isolate) 1 hour (drying) + 10 minutes after inoculation. Reductions are further supported by degradation of RNA on antimicrobial-treated surfaces using qRT-PCR.
Using a polymeric quaternary ammonium antimicrobial (EPA/PMRA registered) the results independently support a sustained antiviral effect via SARS-CoV-2 virion destruction and viral RNA degradation. This indicates that silane-anchored quaternary ammonium compound (SiQAC-18) treated surfaces could play an important role in mitigating the communicability and fomite transmission of SARS-CoV-2.
COVID-19 大流行对全球健康和世界经济造成了前所未有的影响。高毒力和致命性 SARS-CoV-2 变异株的扩散需要有效的传播缓解策略。在合理的环境条件下，可培养和感染性的 SARS-CoV-2 可以在受污染的污染物上存活数小时到数月。在本研究中，我们评估了一种表面锚定的聚合季铵盐抗菌剂，以帮助减少受污染表面 SARS-CoV-2 的污染物传播。
2020 年 3 月，在比利时（雷加医学研究所）和澳大利亚（彼得·多尔蒂感染与免疫研究所）的两个配备有生物安全三级（BSL-3）设施的实验室独立进行了两项研究。这些设施分别于 2020 年 4 月独立应用定量载体为基础的方法，使用真实的 SARS-CoV-2 分离株（hCoV-19/Australia/VIC01/2020、hCoV-19/Belgium/GHB-03021/2020）。
两个设施都独立进行了残留干燥测试，结果表明病毒粒子的破坏持续存在（在接种后 1 小时[干燥]和 10 分钟，GHB-03021 分离株的载量为 108.23 TCID50/载体，VIC01 分离株的载量为 103.66 TCID50/载体）。使用 qRT-PCR 对经抗菌剂处理的表面上 RNA 的降解进一步支持了这些结果。
使用聚合季铵盐抗菌剂（EPA/PMRA 注册），结果独立支持通过 SARS-CoV-2 病毒粒子破坏和病毒 RNA 降解来持续抗病毒作用。这表明，硅烷锚定的季铵化合物（SiQAC-18）处理的表面可能在减轻 SARS-CoV-2 的传染性和污染物传播方面发挥重要作用。
