School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637457, Singapore.
Department of Orthopedic Surgery, College of Medicine, Korea University, 73 Korea-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Adv Sci (Weinh). 2022 Jun;9(18):e2104835. doi: 10.1002/advs.202104835. Epub 2022 Apr 22.
An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource-intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi-quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time.
有效的伤口管理策略需要在整个愈合过程中准确评估伤口状况。这可以通过检查包括蛋白质、DNA 和 RNA 在内的分子生物标志物来实现。然而，目前用于定量这些生物标志物的方法，如免疫组织化学和定量聚合酶链反应，通常既繁琐又耗费资源，且具有破坏性。本文报告了 mRNA 纳米传感器（即 NanoFlare）的开发和利用，该传感器可局部应用于皮肤伤口，通过靶向和半定量检查皮肤细胞中的 mRNA 生物标志物来揭示愈合状态。在 2D 和 3D 体外模型中，这些纳米传感器在揭示不同阶段伤口发展的 mRNA 生物标志物的动态变化方面的功效和效率得到了证明。在小鼠模型中，该平台通过实时的愈合指数，允许对伤口愈合阶段和正常及糖尿病伤口愈合过程进行跟踪和识别。
