Borghi Monica, Perez de Souza Leonardo, Fernie Alisdair R
Department of Biology, Utah State University, Logan, UT, USA.
Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Methods Mol Biol. 2022;2484:3-12. doi: 10.1007/978-1-0716-2253-7_1.
Metabolite profiling aiming at quantifying the metabolome of flowers is emerging as a suitable tool to understand the metabolic complexity of these reproductive organs and the associations between primary and secondary metabolites which characterize them. This chapter provides a general method for the combined analyses of primary and secondary metabolites via gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-mass spectrometry (LC-MS) of flower samples. We describe the preparatory steps, the procedure of metabolites' extraction and finally provide examples of data representation. The method described here can be applied to the analysis of metabolomes of entire flowers, as well as specific flower organs.
旨在定量分析花朵代谢组的代谢物谱分析正成为一种合适的工具，用于理解这些生殖器官的代谢复杂性以及表征它们的初级和次级代谢物之间的关联。本章提供了一种通过气相色谱-质谱联用（GC-MS）和高效液相色谱-质谱联用（LC-MS）对花朵样品中的初级和次级代谢物进行联合分析的通用方法。我们描述了准备步骤、代谢物提取过程，并最终提供了数据呈现的示例。这里描述的方法可应用于对整朵花以及特定花器官的代谢组分析。
