Smith C A, Shingler V, Thomas C M
Nucleic Acids Res. 1984 Apr 25;12(8):3619-30. doi: 10.1093/nar/12.8.3619.
The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD activity. In addition a probable promoter region for the kilB locus was identified. The three promoter regions share common palindromic sequences which may serve as sites for the coordinate regulation of replication and kil functions.
通过RNA聚合酶保护研究确定了广宿主范围IncP质粒RK2（与RP1、RP4、R68和R18相同）的trfA和trfB启动子的位置，并测定了启动子区域的核苷酸序列。trfA启动子序列内的一个突变与kilD活性丧失有关。此外，还确定了kilB基因座的一个可能的启动子区域。这三个启动子区域共享共同的回文序列，这些序列可能作为复制和kil功能协同调节的位点。
