Hall P A, D'Ardenne A J, Butler M G, Habeshaw J R, Stansfeld A G
J Clin Pathol. 1987 Feb;40(2):151-6. doi: 10.1136/jcp.40.2.151.
The use of the murine monoclonal antibody MB2 for identifying B lymphocytes in routinely processed tissue was evaluated and contrasted with the use of the monoclonal antibody UCHL1 for identifying T cells. One hundred and sixty eight surgical biopsy specimens were immunostained with these antibodies, including a wide range of normal and neoplastic non-lymphoid tissues, as well as normal lymphoreticular tissues and lymphomas. Sixty four non-Hodgkin's lymphomas were also examined, of which 51 had been previously phenotypically defined. In selected cases the results were compared with those obtained using two other monoclonal antibodies MB1 and MT1, used for identifying B and T cells, respectively, in paraffin sections. MB1 stained a smaller proportion of B cell tumours than MB2 and staining was, in general, weaker, except in one case of centroblastic lymphoma. MT1 immunoreactivity was comparable with that of UCHL1, except in one case of T lymphoblastic lymphoma (MT1 positive, UCHL1 negative). None of the antibodies is ideal, but, if used as a panel, they permit the separation of B cells and T cells in paraffin sections.
对鼠单克隆抗体MB2用于识别常规处理组织中的B淋巴细胞进行了评估，并与单克隆抗体UCHL1用于识别T细胞的情况进行了对比。用这些抗体对168份手术活检标本进行了免疫染色，包括多种正常和肿瘤性非淋巴组织，以及正常淋巴网状组织和淋巴瘤。还检查了64例非霍奇金淋巴瘤，其中51例先前已进行表型定义。在选定病例中，将结果与使用另外两种分别用于在石蜡切片中识别B细胞和T细胞的单克隆抗体MB1和MT1所获得的结果进行了比较。MB1染色的B细胞肿瘤比例比MB2小，而且一般染色较弱，中枢母细胞性淋巴瘤的一个病例除外。MT1的免疫反应性与UCHL1相当，T淋巴母细胞性淋巴瘤的一个病例除外（MT1阳性，UCHL1阴性）。没有一种抗体是理想的，但如果作为一组使用，它们可以在石蜡切片中区分B细胞和T细胞。
