Xu Lizhou, Al-Jamal Khuloud T
Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.
Institute for Pharmaceutical Science, King's College London, London, UK.
Methods Mol Biol. 2022;2504:21-30. doi: 10.1007/978-1-0716-2341-1_2.
Exosomes are a type of extracellular vesicles that contain constituents including proteins, DNAs, and RNAs of the cells that secrete them. Cancerous exosomes are potential biomarkers for cancer diagnosis. Biosensors are useful analytical tools for quantification of biomarkers and targeted molecules. An aptasensor uses the aptamer as the biorecognition element to bind to the target and is one main type of biosensors that is promising for exosomes analysis and clinical cancer detection. The assay described in this chapter allows for reliable, sensitive, and specific detection of cancer-derived exosomes using a colorimetric aptasensor that is promising for point-of-care testing.
外泌体是一种细胞外囊泡，其包含分泌它们的细胞的蛋白质、DNA和RNA等成分。癌性外泌体是癌症诊断的潜在生物标志物。生物传感器是用于定量生物标志物和靶向分子的有用分析工具。适配体传感器使用适配体作为生物识别元件来结合靶标，是有望用于外泌体分析和临床癌症检测的一种主要生物传感器类型。本章所述的检测方法可使用比色适配体传感器对癌症来源的外泌体进行可靠、灵敏和特异的检测，这种传感器有望用于即时检测。
