Teixeira Marta A, Antunes Joana C, Seabra Catarina L, Tohidi Shafagh D, Reis Salette, Amorim M Teresa P, Felgueiras Helena P
Centre for Textile Science and Technology (2C2T), Department of Textile Engineering, University of Minho, Campus of Azurém, 4800-058 Guimarães, Portugal.
Associate Laboratory for Green Chemistry (LAQV), Network of Chemistry and Technology (REQUIMTE), Departament of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
Int J Biol Macromol. 2022 Jun 1;209(Pt A):1526-1541. doi: 10.1016/j.ijbiomac.2022.04.130. Epub 2022 Apr 22.
In this research, we propose to engineer a nanostructured mat that can simultaneously kill bacteria and promote an environment conducive to healing for prospective wound care. Polyvinyl alcohol (PVA) and cellulose acetate (CA) were combined at different polymer ratios (100/0, 90/10, 80/20% v/v), electrospun and crosslinked with glutaraldehyde vapor. Crosslinked fibers increased in diameter (from 194 to 278 nm), retaining their uniform structure. Fourier-transform infrared spectroscopy and thermal analyses proved the excellent miscibility between polymers. CA incorporation incremented the fibers swelling capacity and reduced the water vapor and air permeabilities of the mats, preventing the excessive drying of wounds. The antimicrobial peptide cys-pexiganan and the immunoregulatory peptide Tiger 17 were incorporated onto the mats via polyethylene glycol spacer (hydroxyl-PEG2-maleimide) and physisorbed, respectively. Time-kill kinetics evaluations revealed the mats effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa. Tiger 17 played a major role in accelerating clotting of re-calcified plasma. Data reports for the first time the collaborative effect of pexiganan and Tiger 17 against bacterial infections and in boosting hemostasis. Cytocompatibility data verified the peptide-modified mats safety. Croslinked 90/10 PVA/CA mats were deemed the most promising combination due to their moderate hydrophilicity and permeabilities, swelling capacity, and high yields of peptide loading.
在本研究中，我们提议设计一种纳米结构垫，该垫能够同时杀死细菌并促进有利于伤口愈合的环境，用于未来的伤口护理。将聚乙烯醇（PVA）和醋酸纤维素（CA）以不同的聚合物比例（100/0、90/10、80/20% v/v）混合，进行电纺丝并与戊二醛蒸汽交联。交联后的纤维直径增加（从194纳米增至278纳米），同时保持其均匀结构。傅里叶变换红外光谱和热分析证明了聚合物之间具有优异的混溶性。掺入CA增加了纤维的溶胀能力，降低了垫子的水蒸气和空气渗透性，防止伤口过度干燥。抗菌肽cys-pexiganan和免疫调节肽Tiger 17分别通过聚乙二醇间隔基（羟基-PEG2-马来酰亚胺）掺入垫子并进行物理吸附。时间杀灭动力学评估揭示了垫子对金黄色葡萄球菌和铜绿假单胞菌的有效性。Tiger 17在加速再钙化血浆的凝血过程中起主要作用。数据首次报道了pexiganan和Tiger 17在对抗细菌感染和促进止血方面的协同作用。细胞相容性数据证实了肽修饰垫子的安全性。交联的90/10 PVA/CA垫子因其适度的亲水性、渗透性、溶胀能力和高肽负载量而被认为是最有前景的组合。
