Centre for Textile Science and Technology (2C2T), Department of Textile Engineering, University of Minho, Campus of Azurém, 4800-058 Guimarães, Portugal.
Associate Laboratory for Green Chemistry (LAQV), Network of Chemistry and Technology (REQUIMTE), Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
Biomater Adv. 2022 Jun;137:212830. doi: 10.1016/j.bioadv.2022.212830. Epub 2022 Apr 29.
Infection is a major issue in chronic wound care. Different dressings have been developed to prevent microbial propagation, but an effective, all-in-one (cytocompatible, antimicrobial and promoter of healing) solution is still to be uncovered. In this research, polyvinyl alcohol (PVA) nanofibrous mats reinforced with cellulose nanocrystal (CNC), at 10 and 20% v/v ratios, were produced by electrospinning, crosslinked with glutaraldehyde vapor and doped with specialized peptides. Crosslinking increased the mats' fiber diameters but maintained their bead-free morphology. Miscibility between polymers was confirmed by Fourier-transform infrared spectroscopy and thermal evaluations. Despite the incorporation of CNC having reduced the mats' mechanical performance, it improved the mats' surface energy and its structural stability over time. Pexiganan with an extra cysteine group was functionalized onto the mats via hydroxyl- polyethylene glycol 2-maleimide, while Tiger 17 was physisorbed to preserve its cyclic conformation. Antimicrobial assessments demonstrated the peptide-doped mat's effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa; pexiganan contributed mostly for such outcome. Tiger 17 showed excellent capacity in accelerating clotting. Cytocompatibility evaluations attested to these mats' safety. C90/10 PVA/CNC mats were deemed the most effective from the tested group and, thus, a potentially effective option for chronic wound treatments.
感染是慢性伤口护理的一个主要问题。已经开发出不同的敷料来防止微生物的传播，但仍然需要找到一种有效的、多合一的（细胞相容性、抗菌和促进愈合）解决方案。在这项研究中，通过静电纺丝制备了聚乙醇酸（PVA）纳米纤维垫，并用 10%和 20%（体积比）的纤维素纳米晶体（CNC）增强，并用戊二醛蒸汽交联，并掺杂了特殊的肽。交联增加了垫子的纤维直径，但保持了无珠的形态。傅里叶变换红外光谱和热评估证实了聚合物之间的混溶性。尽管加入 CNC 降低了垫子的机械性能，但它提高了垫子的表面能和结构稳定性。通过羟基-聚乙二醇 2-马来酰亚胺将带有额外半胱氨酸的 Pexiganan 功能化到垫子上，而 Tiger 17 通过物理吸附来保持其环状构象。抗菌评估表明，肽掺杂垫对金黄色葡萄球菌和铜绿假单胞菌有效；pexiganan 对此结果贡献最大。Tiger 17 在加速凝血方面表现出优异的能力。细胞相容性评估证明了这些垫子的安全性。在测试组中，C90/10 PVA/CNC 垫子被认为是最有效的，因此是慢性伤口治疗的一种潜在有效选择。
