hsa_circ_0000977 的上调通过海绵吸附 miR-874-3p 参与食管鳞癌的进展。
Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR-874-3p.
机构信息
Department of Cardiothoracic Surgery, Lihuili Hospital Affiliated to Ningbo University, Ningbo, China.
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
出版信息
J Clin Lab Anal. 2022 Jun;36(6):e24458. doi: 10.1002/jcla.24458. Epub 2022 Apr 27.
BACKGROUND
Esophageal squamous cell carcinoma (ESCC) is one of the most common clinical malignancies of the digestive system, characterized by high mortality but not evident early symptoms. Molecular markers for diagnostic and outcome prediction are urgently needed. Circular RNAs might play essential roles in the progression of ESCC.
METHODS
Hsa_circ_0000977 was identified using circRNA microarrays and qRT-PCR. The diagnostic value of hsa_circ_0000977 was calculated. We also examined in vitro cell functions in ECA109 and TE12 ESCC cells to determine the effect of hsa_circ_0000977. A dual-luciferase reporter vector validated the binding of hsa_circ_0000977 to miR-874-3p.
RESULTS
The top 10 significantly upregulated circRNAs from microarray assays were hsa_circ_0000977, hsa_circ_0006220, hsa_circ_0043278, hsa_circ_0000691, hsa_circ_0000288, hsa_circ_0000367, hsa_circ_0021647, hsa_circ_0006440, hsa_circRNA_405571 and hsa_circRNA_100790, while the top 10 significantly downregulated circRNAs were hsa_circ_0008389, hsa_circ_0089763, hsa_circ_0089762, hsa_circ_0000102, hsa_circ_0001714, hsa_circ_0089761, hsa_circ_0007326, hsa_circ_0001549, hsa_circ_0005133 and hsa_circRNA_405965. Hsa_circ_0000977 was significantly upregulated in ESCC (p < 0.01) and had diagnostic value in ESCC. The hsa_circ_0000977 expression level was related to the pT stage and numbers of lymph nodes in ESCC patients. Elevated hsa_circ_0000977 promoted cell proliferation, migration and inhibited apoptosis in ESCC cells. Hsa_circ_0000977 might function as a micro-RNA sponge to competitively bind miR-874-3p.
CONCLUSION
Disordered hsa_circ_0000977 expression can promote carcinogenesis in ESCC and might serve as a diagnostic biomarker to evaluate the occurrence and development of esophageal cancer.
背景
食管鳞状细胞癌（ESCC）是消化系统最常见的临床恶性肿瘤之一，其死亡率高，但早期症状不明显。因此，急需用于诊断和预后预测的分子标志物。环状 RNA 可能在 ESCC 的进展中发挥重要作用。
方法
使用 circRNA 微阵列和 qRT-PCR 鉴定 hsa_circ_0000977。计算 hsa_circ_0000977 的诊断价值。我们还在 ECA109 和 TE12 ESCC 细胞中进行了体外细胞功能研究，以确定 hsa_circ_0000977 的作用。双荧光素酶报告载体验证了 hsa_circ_0000977 与 miR-874-3p 的结合。
结果
微阵列分析中前 10 个显著上调的 circRNAs 是 hsa_circ_0000977、hsa_circ_0006220、hsa_circ_0043278、hsa_circ_0000691、hsa_circ_0000288、hsa_circ_0000367、hsa_circ_0021647、hsa_circ_0006440、hsa_circRNA_405571 和 hsa_circRNA_100790，而前 10 个显著下调的 circRNAs 是 hsa_circ_0008389、hsa_circ_0089763、hsa_circ_0089762、hsa_circ_0000102、hsa_circ_0001714、hsa_circ_0089761、hsa_circ_0007326、hsa_circ_0001549、hsa_circ_0005133 和 hsa_circRNA_405965。hsa_circ_0000977 在 ESCC 中显著上调（p<0.01），并具有 ESCC 的诊断价值。hsa_circ_0000977 的表达水平与 ESCC 患者的 pT 分期和淋巴结数量有关。升高的 hsa_circ_0000977 促进 ESCC 细胞的增殖、迁移和抑制凋亡。hsa_circ_0000977 可能作为 micro-RNA 海绵与 miR-874-3p 竞争结合。
结论
失调的 hsa_circ_0000977 表达可促进 ESCC 发生癌变，可能作为评估食管癌发生和发展的诊断生物标志物。
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